广东地区人禽流感H5N1血凝素基因特征与进化.docVIP

广东地区人禽流感H5N1血凝素基因特征与进化 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：广东地区人禽流感H5N1血凝素基因特征与进化 1 1 材料与方法 3 2 结果 4 3 讨论 6 文2：广东地区人禽流感H5N1血凝素基因特征与进化 7 1 材料与方法 8 2 结果 9 3 讨论 12 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 14 正文 广东地区人禽流感H5N1血凝素基因特征与进化 文1：广东地区人禽流感H5N1血凝素基因特征与进化 Characteristic and evolution on HA gene of human H5N1 avian influenza Abstract： Objective To reveal characteristic and evolution on HA geoe of human H5N1 avian influenza by analyzing variation of the nucleotide sequence of HA gene of human H5Nl avian HA gene of Guangdong strain was sequenced and othe global HA nucleotide sequences of human H5Nl avian influenza from Internet and their variation were clustered and compared and analyzed with and DNA Forty-two HA genes of human H5N1 avian influenza in 1997～2006 were clustered into three ～nine amino acids of HA gene were substituted and accounted for ％(89／568).HA gene increased one glycoprotein domain in 170～172 sites(T／S) in 2003～ the synonymous varimion，Ks in HA gene was between ×10-5～×10-5 and the evolutionary linear regression equation was Y=×10-9X+×，Ks was more than Ka in same strain and Ks／Ka value showed that there was not selective pressure in HA gene strain evolution was divided into three cluste，one for strai in 1997～1998，another for strai in 2003～2005 except for strai from China，third for strain from China in 2005～ variation of strain HK-213-03 showed their traitio between 1997 and 2004,and the variatio of the amino acids on HA in TL-L2004-04 was There was the differences between HA of strai in 2003～2006 and strai in 1997 and one more glycoprofein domain might increase the viral pathogenicity；it was worth noticing variatio of the amino acids on HA in H5N1 strai evolve frequently in the natural environment,but were not subject to avian or human immunological ，the probabilities were great that H5N1 strai possess of human-to human tramittion as the natural antigenicity of HA gene was needed to coider in vaccine of diff