卡配因I细胞周期依赖性蛋白激酶5通路在血管性痴呆小鼠海马中的变化临床医学.docVIP

卡配因I细胞周期依赖性蛋白激酶5通路在血管性痴呆小鼠海马中的变化临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：卡配因I细胞周期依赖性蛋白激酶5通路在血管性痴呆小鼠海马中的变化临床医学 1 1 材料与方法 3 2 结 果 5 3 讨 论 6 文2：血管性痴呆小鼠海马NOS活力和nNOS蛋白表达的改变医学 8 1材料和方法 8 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 卡配因I细胞周期依赖性蛋白激酶5通路在血管性痴呆小鼠海马中的变化临床医学 文1：卡配因I细胞周期依赖性蛋白激酶5通路在血管性痴呆小鼠海马中的变化临床医学 【Abstract】 Objective To observe the changes of the expression of Calpain I cyclindependent protein kinase 5 (Cdk5) pathway at different time points following cerebral ischemia and reperfusion. Methods 160 male Kunming mice were randomly divided into sham and model groups. Vascular dementia (VD) mice model was made by three times of traient ischemia and reperfusion of bilateral common carotid arteries. The behavioral tests were examined respectively at 4, 6 w post surgery. The expression of Calpain I in hippocampal CA1 was measured by immunohistochemistry staining. Cdk5 mRNA was examined by RTPCR. Western blot was used to evaluate Cdk5 protein expression. Results Compared with those in sham group， the abilities of learning and memory of animals in model group were damaged significantly(P) at 4, 6 w post surgery. At 4 w，the OD value of Calpain I in model group was higher than that of sham group( 0± 0 vs 2± 3), and there was significant differene between the two groups(P). The expression of Cdk5 mRNA in model group was higher than that of sham group(± vs ± ，P). At 6 w，the OD value of Calpain I in model group was higher than that of sham group( 0± 4 vs 7± 5，P). The expression of Cdk5 mRNA in model group was also significantly higher than that of sham group(± vs ± ，P). Conclusio Calpain I Cdk5 pathway is involved in the occurrence of VD in mouse following cerebral ischemia and reperfusion. 【Key words】 Ischemia and reperfusion;Vascular dementia;Calpain I ;Cyclindependent protein kinase 5 细胞周期依赖性蛋白激酶5( Cyclindependent protein kinase 5，Cdk5)是细胞周期依赖性激酶家族中的一员，研究表明Cdk5通过磷酸化其底物，控制多种激酶和信号转导通路，参与学习和记忆的形成〔1〕，对脑的发育和功能具有重要意义。除正常的生理功能外，Cdk5还参与了多种神经变