探讨男性染色体结构与数目异常（教学资料）.docVIP

探讨男性染色体结构与数目异常（教学资料） 文档信息 ： 文档作为关于“中学教育”中“中学课件”的参考范文，为解决如何写好实用应用文、正确编写文案格式、内容素材摘取等相关工作提供支持。正文9643字，doc格式，可编辑。质优实惠，欢迎下载！ 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：探讨男性染色体结构与数目异常 1 1 对象与方法 3 2 结果 5 3 讨论 7 文2：关于生精汤治疗顶体酶异常男性少弱精症疗效观察 9 1 材料与方法 10 2 结果 11 3 讨论 12 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 探讨男性染色体结构与数目异常（教学资料） 文1：探讨男性染色体结构与数目异常 Study of the genetic etiology of male infertility WANG Tian-yuan XIAO Xiao-su ZHENG-Bao HAO Ya-jun LIU Shi-shi Clinical Laboratory， Nahan District People′s Hospital Genetic Definition Clinic， Shenzhen Hospital of Peking Univeity Abstract： Objective:To investigate the correlation of male infertility with abnormality of chromosomal cotruction and quantity and with the microdeletion of azoospermia factor (AZF) gene on the Y chromosome. As well as the relatiohip between the partial deletio in the AZFc region of Y chromosome and male infertility. Methods:Multiplex-PCR technique was used to detect microdeletio of AZF gene in 494 infertile men and 236 fertile controls with fifteen sequence-tagged sites (STSs) . Chromosome karyotype analyses were performed in the 494 patients. Results:In the azoospermia， severe oligozoospermia and oligozoospermia patients， the incidences of chromosomal abnormality were % (21/177) 、% (6/177) 、% (3/144) .The rates of AZF microdeletion in azoospermia and severe oligozoospermia were significant higher than that of the oligozoospermia. there were significant differences in the b2/b3 deletion between patients with spermatogenic failure and serious oligozoospermia and controls (both P Keyword： Chromosomal abnormality; AZF microdeletion; AZFc region; Male infertility; Received： 2017-10-20 不孕不育症是一类严重危害人类身心将健康的疾病， 其发生占育龄夫妇的10%左右， 其中男性因素导致不孕不育的占50%。男性不育的病因复杂， 主要表现为生精障碍所致的无精子症、严重少精子症和少精子症。目前大量研究认为许多因素与男性不育相关， 如性腺功能低下、精索静脉曲张、生殖泌尿道感染、输精管梗阻、隐睾、获得性睾丸炎等。由于遗传缺陷包括染色体异常和基因突变所引起的精子发生障碍约占男性不育的30%以上[1]。本实验通过较大样本量的病例对照研究 (177例无精子症患者， 177例严重少精子症患者， 140例少精子症患者和236例精液正常已生育男性) ， 探讨染色体结构与数目异常， 以及Y染色体无精子因子 (a