单麻叶千里光化学成分研究.docx

单麻叶千里光化学成分研究 senamifoliusvar.位于局部，被称为“fluenti”，主要位于中国、韩国、日本和东部城市。 In this paper, we reported the isolation and structure elucidation of nine compounds from the ethanol extract of S.cannabifolius var.integrilifolius.These compounds were identified as:sugiol (1) , zhebeiresinol (2) , lirriodendrin (3) , oleanane (4) , 1, 4-benzenediol (5) , ethyl 2, 5-dihydroxy benzeneacetate (6) , p-hydroxyphenylacetylamide (7) , quercetin (8) and hyperoside (9) .Among them compounds 1and 2 were found from the genus for the first time, and compounds 1~9 were all isolated from the plant for the first time. 1 pharmaceutity.3.4.3.3cificiding Melting points were determined using an X-4 digital micromelting point apparatus (uncorrected) . Senecio cannabifolius var.integrilifolius was offered by Xiuzheng Pharmaceutical Co.Ltd.and identified by Prof Zhang Mian (Department of Pharmacognosy, China Pharmaceutical University) .A voucher specimen (No.DMYQLG-2004-12) was deposited in the Department of Pharmacognosy, China Pharmaceutical University. 2 规训acid-int Air-dried and powdered aerial parts of S.cannabifolius var.integrilifolius (45 kg) was refluxed with 90%ethanol and concentrated.The extract (4 kg) was dissolved in 2%HCl and filtrated.Then the acid-insoluble part was partitioned by Et OAc and n-Bu OH successively.Et OAc fraction (200 g) was subjected to repeated silica gel and Sephadex LH-20 column chromatography, and further purified by recrystallization.At last compound 1 (15 mg) , 2 (12mg) , 3 (20 mg) , 4 (10 mg) , 5 (30 mg) , 6 (25 mg) and 8 (15mg) were obtained.By the same means, 7 (100 mg) and 9 (40 mg) were isolated from n-Bu OH fraction (400 g) successfully. 3 etoac-hex现行标准amesi-ms-z303. Compound 1 white crystal (Me Compound 2 white crystal (Hexane-Me Compound 3 white crystal (Hexane-Me Compound 4 white crystal (Et OAc-Hexane) , mp209~210°C.It was characterized as oleanane by the comparision of the physical and spectral data with the reported data Compound 5 white crystal (Et OAc