用于SRAS冠状病毒SNP位点检测与分型芯片的研制.docVIP

  Development of a single nucleotide polymorphism (SNP) DNA Microarray for Detection and Genotyping of SARS  5 10 15 20 25 30 35 40 Coronavirus GUO Xi, LIU Bin** (TEDA School of Biological Sciences and Biotechnology, Nankai University, TianJin 300457) Abstract: A novel coronavirus, severe acute respiratory syndrome coronavirus (SARS-CoV), has been identified as the causative agent of severe acute respiratory syndrome. It has been known that the spike (S) glycoprotein is required for both viral attachment to its permissive cells and fusion of the viral envelope with the host cell membrane. There are 27 single nucleotide variations(SNVs) in the s gene encoding for the spike glycoprotein which are maybe related to the epidemic of this virus[10]. In our study, a SNP detecting microarray system including 108 probes was developed and the utility of this system was demonstrated in the parallel analysis of 25 point mutations from s gene. The efficiency and specificity of this microarray was evaluated by analysis of 20 samples and the veracity was 95%, this result showed that our microarray may be a promising tool for SARS-CoV detection and genotyping and analysis of its prevalence. Keywords: SARS Coronavirus; Single Nucleotide Polymorphism; DNA Microarray 0 Introduction The causative agent of the outbreak of the atypical pneumonia known as Severe Acute Respiratory Syndrome (SARS) has been identified as a novel coronavirus (CoV)[1-3]. Phylogenetic analysis of the SARS-associated CoV (SARS-CoV) shows that it is neither a mutant nor a recombinant of previously characterized CoV, and forms a new, distinct group within the genus[4]. The exact origin of the cause of the Severe Acute Respiratory Syndrome (SARS) is still an open question. Before long the global outbreak of SARS, SARS-CoV like viruses have been detected in game animals that are commonly sold in Chinese markets , and it is probable that the virus replicates in an animal reservoir[5-6]. Spike(S) protein is one of