具有酯酶活性的小分子水凝胶.docVIP

  具有酯酶活性的小分子水凝胶# 杨成彪1，王怀民1，王玲2，李东霞1，杨志谋1,3** （1. 南开大学生命科学学院，生物活性材料教育部重点实验室，天津 300071； 5 10 15 20 25 30 35 40 2. 南开大学药学院，天津市分子药物研究重点实验室，天津 300071； 3. 南开大学药物化学国家重点实验室，天津 300071） 摘要：仿生的小分子水凝胶研究领域吸引了研究人员的广泛兴趣。本文报道了几种基于含有 L-组氨酸的多肽小分子水凝胶, 其通过加热-冷却循环形成。对比于 L-组氨酸, 由于自组装纳 米纤维的表面有高密度的 L-组氨酸残基,所有水凝胶表现出更高的水解 4 - 硝基苯基乙酸乙 酯（4-NPA）的活性。为了计算 4-NPA 水解率，我们建造了米氏酶的动力学模型。在这些 凝胶中，Nap-GFFYGHY 的凝胶具有最高的使酯键断裂的人工酶活性，约是对照的 25 倍。 这些结果表明，由自组装纳米纤维形成的小分子水凝胶在自组装的仿造人工酶领域有很大的 潜力。 关键词：自组装；水凝胶；人工酶 中图分类号：R318 Molecular hydrogels with Esterase-like activity Yang Chengbiao1, Wang Huaimin1, Wang Ling2, Li Dongxia1, Yang Zhimou1,3 (1. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, TianJin 300071; 2. College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, TianJin 300071; 3. State Key Laboratory of Medicinal Chemical Biology, Nankai University, TianJin 300071) Abstract: The development of molecular hydrogels that can be applied for mimicking bioactive molecules attracts extensive interests for researchers in fields of self-assembly. In this study, we reported on several molecular hydrogels based on naphthylacetic acid-peptides containing L-histidine formed by the heating-cooling process. All hydrogels exhibited higher activity to hydrolyze 4-nitrophenyl acetate (4-NPA) than the free L-histidine probably due the high density of L-histidine residue at the surface of self-assembled nanofibers. To calculate the 4-NPA hydrolysis rates, the Michaelis-Menten enzyme kinetics model was built. Among these gels, the gel of Nap-GFFYGHY possesses the highest enzyme activity of making the ester bond cleavage, which is approximately 25 times higher than that of control. The results indicate that molecular hydrogels with self-assembled nanofibers have great potentials for the generation of self-assembled multivalent materials. Keywords: self-assembly; hydrogel; artificial enzyme 0 引言 对化学催化和酶催化的深入了解已经促使研究人员尝试利用纳米结构作为人工酶[1-6]。 为了发展具有高催化活性模拟酶的纳米结构，在纳米结构表面构建高密度的人工活性