甘草苷、甘草次酸与次乌头碱配伍的研究.docVIP

  甘草苷、甘草次酸与次乌头碱配伍的研究 刘巧云，张宇燕，万海同，周天梅，杨珍** （浙江中医药大学心脑血管病研究所，杭州 310053） 5 10 15 20 25 30 35 40 45 摘要：目的:探讨甘草苷、甘草次酸与次乌头碱(HA)配伍对心肌细胞 MDA 含量及心肌连接 蛋白 43(CX43)表达水平的影响。方法：以新生 SD 乳鼠心肌细胞为研究对象， HA 分别单 独作用，与甘草苷、甘草次酸、甘草苷+甘草次酸不同浓度按 1:1、1:2、1:4 比例配伍作用于 心肌细胞，检测其 MDA 活性，根据结果选取配伍效果理想的配伍组,采用 RT-PCR 测定心 肌连接蛋白 43(CX43)基因表达。结果：HA 与甘草苷、甘草次酸、甘草苷和甘草次酸配伍组 比 HA 单独作用可以增加心肌细胞活力,减少 MDA 含量,其中 1:1 酸乌、1:4 苷乌组、1:1:1 酸 苷乌组作用可以增加 CX43 基因的表达水平。结论：适宜浓度比例的甘草苷、甘草次酸与 HA 配伍可以减少 HA 对心肌细胞的氧化损伤及增加 43(CX43)基因表达水平。 关键词：次乌头碱；甘草苷；甘草次酸；心肌细胞；MDA；CX43 基因 中图分类号：R284 Study of the compatibility of hypaconitine and liquiritin 、 glycyrrhetinic acid LIU Qiaoyun, ZHANG Yuyan, WAN Haitong, ZHOU Tianmei, YANG Zhen (Institute of Cardio-Cerbrovascular Disease, Zhejiang Chinese Medical University, HangZhou 310053) Abstract: Objective：To explore the compatibility of hypaconitine and liquiritin 、glycyrrhetinic acid which effect content of MDA and expression leve1 of connexin43(Cx43) gene to myocardial cells. Methods：Using the primary cultured myocardial cells as reseach subjects, hypaconitine work and the proportion of aconitine to liquiritin and glycyrrhetinic acid are 1:1、1:2、1:4 respectively groups to myocardial cells, detect content of MDA.then according this result,selecting groups works best.test leve1 of connexin43(Cx43) gene by RT-PCR.Results：compatibility of hypaconitine and liquiritin,hypaconitine and glycyrrhetinic acid ，hypaconitine and liquiritin、 glycyrrhetinic acid decrease content of MDA and elevate leve1 of connexin43(Cx43) gene than only hypaconitine.especially the compatibility of hypaconitine and liquiritin(1:4) 、 the compatibility of hypaconitine and glycyrrhetinic acid (1:1)、the compatibility of hypaconitine and liquiritin、glycyrrhetinic acid (1:1:1). Conclusions：proportion of appropriate concentration of compatibility of hypaconitine and liquiritin 、 glycyrrhetinic acid can reduce oxidation and damage of myocardial cells and elevate leve1 of connexin43(Cx43) gene. Keywords: Hypaconitine; Liquiritin; Gly