利用Solexa测序技术发掘银杏microRNA.docVIP

  利用 Solexa 测序技术发掘银杏 microRNA# 张倩，邢世岩** （山东农业大学林学院，山东 泰安 271018） 5 10 摘要：microRNA( miRNA)主要是在转录后水平上通过介导靶基因 mRNA 的裂解或抑制翻译 来调控生物基因的表达，在植物生长发育的和胁迫响应等方面发挥着重要调控作用。本研究 利用基于 Solexa 原理的小分子 RNA 深度测序技术，并结合生物信息学的方法对胚珠发生时 期的银杏叶片中保守和新预测的 miRNA 类型、表达丰度及靶基因进行了分析。研究共发现 隶属于 23 个 miRNA 家族的 80 个保守的 miRNA，其表达丰度在 1~338015 之间，其中包 含一个可能为裸子植物特有的 miR1314。此外还预测 6 条新 miRNA，其中两个为多基因座 miRNA。靶基因预测发现功能涉及转录调控、胁迫响应和信号转导等过程，为研究 miRNA 在银杏胚珠发生和发育过程中的调控机理奠定良好的基础。 关键词：银杏；Solexa 高通量测序；microRNA；靶基因 中图分类号：S664.3 15 Novel and conserved microRNAs identification by Solexa sequencing in Ginkgo biloba L. ZHANG Qian, XING Shiyan (Colleage of Forestry, Shandong Agricultural University, ShanDong TaiAn 271018) 20 25 30 35 40 Abstract: microRNA( miRNA) plays a very important part in plant biological development and responsing to stress , which negatively regulates gene expression at the posttranscriptional level by targeting mRNAs for cleavage or repressing translation. Combined with bioinformatic method, the types along with abundance and targets of known and novel miRNAs in Ginkgo biloba L. were analyzed by small RNA deep sequencing technology, which was based on Solexa principium. The results indicated 80 conserved miRNA sequences belonging to 23 miRNA families, with abundance from 1 to 338015.Besides miR1314 may be one of the special gymnosperms miRNAs. In addition 6 new miRNAs were also predicted, two of which were loci miRNAs. Target gene prediction showed that they were involved in the following processes, such as transcription regulation, substance and energy metabolism and signal transduction. In conclusion, this research made a good foundation for miRNA regulation mechanism in the ovule occurrence and development process. Keywords: Ginkgo biloba L.; Solexa sequencing; MicroRNA; Target genes 0 引言 MicroRNA（miRNA）作为一类长度在 18～25 个碱基之间的非编码负调控因子，主要 在转录后水平上通过介导靶基因 mRNA 的降解或抑制翻译过程来调节基因的表达[1-4]。越来 越多的 miRNA 研究表明，miRNA 通过调节转录因子、信号胁迫相关蛋白及代谢相关的酶 等靶标，在植物的组织分化、器官的极性发育和形态建成、营养生长向生殖生长的转变以及 激素的信号转导、胁迫应答、抗性等生命进程的各个方面