骨髓间充质干细胞向成骨细胞分化的影响因素.docVIP

  骨髓间充质干细胞向成骨细胞分化的影响 因素# 管常东，黄艳，樊瑜波** 5 10 （北京航空航天大学生物与医学工程学院，生物力学与力生物学教育部重点实验室， 北京 100191） 摘要：背景：目前以骨移植为基本理念的骨组织工程在骨损伤临床治疗中被广泛应用，骨髓 间充质干细胞作为骨组织工程研究领域最重要的种子细胞，其向成骨细胞方向分化的诱导成 为该领域的一个研究热点。研究现状：许多研究已经证实了在体外可以诱导骨髓间充质干细 胞向成骨细胞方向分化，当前的研究热点集中在该诱导条件的优化，以期体外获得大量性状 稳定的成骨细胞。研究用途：有助于解决骨组织工程中细胞来源的问题，在骨科疾病的临床 治疗领域有重要的研究意义。 关键词：生物医学工程；骨髓间充质干细胞；成骨细胞；分化；生物化学因素；物理因素 中图分类号：R318 15 Influence Factors on Osteogenic Differentiation of Bone Marrow-derived Mesenchymal Stem cells GUAN Changdong, HUANG Yan, FAN Yubo (Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of 20 25 30 35 40 Biological Science and Medical Engineering, Beihang University, Beijing 100191) Abstract: Background: with the basic concept of bone graft, bone tissue engineering was widely used in the field of bone injury clinical treatment. Bone marrow mesenchymal stem cells (BMSCs) were shown as the most important seed cells of bone tissue engineering, and received extensive attention because they could be induced to differentiate into osteoblasts. Research progresses: many studies have already confirmed that BMSCs could be induced to differentiate into osteoblasts in vitro, nowadays many researchers focus on the optimization of the conditions during the process of induction, in order to get numerous osteoblasts. Research purposes: the aim of those studies were to get enough cells for bone tissue engineering, which showed significance in the field of clinical treatment for orthopedic diseases. Keywords: Biomedical engineering; Bone marrow-derived mesenchymal stem cells; Osteoblasts; Differentiation; Biochemical factors; Physical factors 0 引言 骨髓间充质干细胞（bone marrow-derived mesenchymal stem cells，BMSCs）是源于骨髓 的多能干细胞，因具有取材容易、机体损伤小、易于体外培养增殖、有多向分化潜能、组织 相容性好、易于外源基因的导入等优点，成为组织工程和基因工程理想的靶细胞，在细胞生 物学、临床医学等领域有着越来越重要的研究价值。体外培养条件下，BMSCs 容易被多种 环境因素诱导分化，不同的环境因子可诱导 BMSCs 向成骨细胞、脂肪细胞、骨骼肌细胞、 软骨细胞、平滑肌细胞、血管内皮细胞、神经元细胞、心肌细胞等方向分化。随着成体干细 胞研究的不断普及，BMSCs 在干细胞研究领域展现了越来越重要的价值。 骨组织工程需要大量的种子细胞，近年来国内外学者和临床工作者对诱导干细胞成骨向