磷和不同价态无机砷对蜈蚣草砷磷吸收和根系分泌物的影响.docVIP

  磷和不同价态无机砷对蜈蚣草砷磷吸收和 根系分泌物的影响# 娄来清，史高玲，蔡庆生* 5 10 15 20 25 30 35 40 （南京农业大学生命科学学院） 摘要：利用溶液培养的方法研究了蜈蚣草对 As（V）和 As（III）的吸收差异及砷和磷之间 的相互作用。研究结果表明，蜈蚣草对砷的吸收受到营养液中砷的化学形态和浓度的影响。 与 As（III）处理相比，As（V）处理条件下，蜈蚣草对砷的吸收和向地上部分转运的能力 明显增强。P 的处理可以减少蜈蚣草对砷的吸收，对 As（V）的影响尤其明显，同样，砷处 理减少了根系对 P 的吸收。As（V）处理下，蜈蚣草根系、地上部的 As 和 P 含量之间都存 在着负相关性，这种负相关性同样存在于 As（III）处理下蜈蚣草的根系中。蜈蚣草中砷向 地上部的转运受砷的形态和磷的共同影响，表现为 As（V）胁迫下，砷在蜈蚣草体内的转 运系数明显比 As（III）胁迫下砷的转运系数高，加磷时砷的转运系数降低。加砷和缺磷处 理会增加根系分泌物中可溶性有机碳含量（DOC）。 关键词：As（III）；As（V）；蜈蚣草；转运；DOC 中图分类号：Q89 The Effects of Phosphate on As Uptake and DOC Contents in Root Exudates of Chinese brake fern under Different Arsenic exposure LOU Laiqing, SHI Gaoling, CAI Qingsheng (Collge of Life Sciences of Nanjing Agricultural University) Abstract: Hydroponic cultures were used to study the differences of arsenate and arsenite uptake and translocation in Chinese brake fern were studied under the influence of phosphate. The results showed that As uptake was significantly influenced by both the chemical form and concentration of As in the nutrient solution. Higher abilities of As uptake and translocation in plants treated with arsenate were observed. Phosphate Phosphate inhibited As uptake and transport in Chinese brake fern, especially for arsenate treatments. Arsenic concentrations were negatively correlated with P in roots and fronds of Chinese brake fern treated with arsenate, as well as in roots of plants treated with arsenite. Transport factors (TFs) of As were affected by As species and P, higher TFs were observed in Chinese brake fern exposed to arsenate than those of arsenite. The addition of higher concentrations of As and deficiency of P in nutrient solution resulted in the increase in dissolved organic carbon (DOC) contents in root exudates of Chinese brake fern. Key words: arsenite; arsenate; Pteris vittata; transport; dissolved organic carbon 0 引言 砷（As）是环境中重要的污染物之一。由于矿山的开采和冶炼，以及矿区附近废水的 不合理排放，环境中的砷（As）污染日益严重。无机砷（As（III）、As（V））是土壤和地 下水中砷存在的主要形式，且对植物的毒性较大[1; 2]。在有氧的条件下，土壤中