激活Rho激酶对大鼠海马神经元突起生长影响的 原子力显微镜观察.pdfVIP

Rho ,Rho LPA NeurobasalB27 6-11d 48 LPA 48 Rho Rho Rho 1 ABSTRACT Objective: To invesitgate the process of neurites collapsion by activating the Rho kinase pathway, at the same time observe the changes of the cell membrane structure to enrich the morphology data in neurites formation. Materials and methods: Cultured rat hippocampal neurons were treated with the accelerator of Rho kinase-LPA. The process of neurites extension and tendency of cell membrane changes were analyzed by atomic force microscopy. Results: Neurobasal with B27 has shown excellent long-term viability and nearly pure of postnatal rat hippocampal neurons. When culturing during 6-11 days, hippocampal neurons can be adapted for some other experiments like construction and function. With the development of neurons, the neurites were growing continously and branches getting more and more, the length, diameter and number of the neurons were getting the growth peak when they were cultured 48 hours, and getting declined later. Distinct neurites, enriched cell membrance structures incl-uding membrance proteins and hole-like structures were ob