牛血清蛋白壳聚糖自组装纳米胶束的制备及理化性质研究.pdfVIP

牛血清蛋白壳聚糖自组装纳米胶束的制备及理化性质研究1 * ， 霍美蓉，周建平 陆建男，钱红艳 【摘 要】 目的：两亲性壳聚糖衍生物N- 辛基-O, N-羟乙基壳聚糖(OGC)对牛血清蛋 白（BSA）的负载及物化性质研究。方法：采用超声法制备BSA 自组装纳米胶束（BSA-OGC 纳米胶束），高速冷冻离心和荧光法测定包封率和载药量，并测定粒径、zeta- 电位，透射电 镜 （TEM ）观察其形态，荧光扫描、红外光谱 （FT-IR）、差示扫描量热分析 （DSC）和广 角 x-粉末衍射 （WARD）研究其理化性质。结果：BSA-OGC 包封率、载药量分别为 42～ 81% 、 11～4.85% ；载药后胶束粒径由 194.8±6.0nm 降至 160.3±8.9nm～186.6±7.9nm，zeta- 电位由 16.8±3.8 mV 降至 3.3±0.2 mV～8.2±0.3 mV；TEM 照片显示OGC 和BSA-OGC 皆 为规则球体，粒径分布均匀；荧光扫描、FT-IR、DSC、WARD 结果表明BSA 包裹在OGC 中，证明两者是通过电荷和疏水双重作用自组装成纳米胶束。结论：两亲性壳聚糖衍生物 （OGC ）作为蛋白类药物的传递载体具有潜在应用价值。 【关键词】：牛血清蛋白；壳聚糖；自组装；纳米胶束 Preparation and characterization of Bovine Serum Albumin-loaded chitosan self-assembled nanomicelles 【ABSTRACT 】 AIM: Bovine Serum Albumin-loaded N-octyl-O,N-hydroxyethyl chitosan self- assembled nanomicelles (BSA-OGC) were prepared and characterized. METHODS: BSA-OGC nanomicelles were prepared and The free protein and nanomicelles were separated by high-speed refrigeration centrifugation. Drug loading efficiency and loading capacity were measured by fluorescence, the diameter and zeta potential of nanomicelles were measured and BSA-OGC were characterized by transmission electron microscopy (TEM) ,fluorescence spectrum,fourier transformed infrared spectrophotometer (FT-IR), differential scanning calorimetry (DSC) and wide angle X-ray diffraction (WAXD). RESULTS: Loading efficiency and loading capacity of BSA-OGC were (42.09±1 .17) ～(81.53±2.45) % and ( 10.97±2.25) ～(4.85±0.03) wt. %, respectively. The diameter of BSA-OGC was (160.3±8.9) nm～(186.6±7.9) nm with zeta potential (+3.3±0.2) mV～(+8.2±0.3) mV compared to OGC (165.6±10.7) nm with (+16.8±3.8) mV. TEM images of OGC and BSA-OCC showed a spherical shape. Fluorescence spectrum,FT- IR,DSC,WAXD of OGC-BSA indicated that BSA-loaded OGC nanomicelles were self-assemb