植物疫苗植物疫苗无致病力青枯雷尔氏菌胁迫对烟草根系土壤微生物脂肪酸生态学特性与影响.docVIP

植物疫苗植物疫苗无致病力青枯雷尔氏菌胁迫对烟草根系土壤微生物脂肪酸生态学特性的影响 郑雪芳，刘波(，蓝江林，朱育菁，车建美，苏明星 （福建省农业科学院农业生物资源研究所，福州 350003） 摘 要：为了探讨植物疫苗无致病力青枯雷尔氏菌对烟草根系土壤微生物群落结构的影响，本研究测定了植物疫苗无致病力青枯雷尔氏菌和清水对照的烟草根系土壤Phospholipid Fatty Acid, PLFAs），比较分析植物疫苗无致病力青枯雷尔氏菌和对照烟草根系土壤微生物PLFAs组成、含量、微生物群落结构及多样性的差异，从微生物群落水平解析植物疫苗无致病力青枯雷尔氏菌对烟草青枯病的免疫机制。结果表明，与对照相比，植物疫苗无致病力青枯雷尔氏菌烟草根系土壤类型，10Me16:0含量降低，为下降型，增100%以上类型的PLFAs均指示革兰氏阴性菌。植物疫苗无致病力青枯雷尔氏菌能改变烟草根系土壤微生物群落结构，促进细菌和真菌的生长，抑制放线菌的生长。Simpson、Shannon和均匀Pielou指下烟草根系土壤微生物聚类分析表明，基本上可将植物疫苗无致病力青枯雷尔氏菌和清水对照烟草根系土壤分别聚在两不同的类群中，说明植物疫苗无致病力青枯雷尔氏菌能明显改变根系土壤微生物群落结构。 关键词：植物疫苗无致病力青枯雷尔氏菌；根系土壤微生物群落结构；磷脂脂肪酸 Effect of the plant vaccin of avirulent strain Ralstonia solanacearum on soil microbial community PLFAs structure in the rhizosphere of tobacco ZHENG Xue-fang, LIU Bo*, LAN Jiang-lin, ZHU Yu-jing, CHE Jian-mei, SU Ming-xing Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fujian Fuzhou 350003 Abstract: In order to study the effects of avirulent Ralstonia solanacearum on soil microbial community structures in the root system, the phospholipid fatty acids (PLFAs) from the samples of tobacco in the different date were detected.. The pattern, content and structure of PLFAs in the treatment with plant vaccin of avirulent strain R. solanacearum and in the treatment with water control were compared.The results showed that plant vaccin of avirulent strain R. solanacearum stress changed PLFAs pattern and content of tomato root soil, which could divided five groups that declined type, unchanged type, increased 50% type, increased 50-100% type, and inceaded more than 100% type. Moreover, plant vaccin of avirulent strain R. solanacearum treatment changed the diversity of microbial comunities in the tobacco rhizosphere, that promoted the growth of bacteria and fungi, inhibited the growth of actinomycetes. We also found that plant vaccin of avirulent strain R. solanacearum could increased the community diversity of tobacco rhizosphere when based on the indexes of Shannon,