柔红霉素（DNR）荧光直方图诊断白血病细胞耐药的意义.docVIP

柔红霉素（DNR）荧光直方图诊断白血病细胞耐药的意义 【摘要】目的：检测细胞内柔红霉素（DNR）的荧光直方图诊断白血病细胞耐药的方法。材料与方法：选取15例临床耐药的急性髓性白血病（AL），5初治AL，用流式细胞仪（F）检测细胞内DNR的荧光强度，其强弱程度划分成耐药区和药… 【摘要】目的：检测细胞内柔红霉素（DNR）的荧光直方图诊断白血病细胞耐药的方法。材料与方法：选取15例临床耐药的急性髓性白血病（AL），5初治AL，用流式细胞仪（F）检测细胞内DNR的荧光强度，其强弱程度划分成耐药区和药敏区，其直方图主峰左移（LSP）诊断为耐药；直方图右移（RSP）诊断为药敏。耐药指数（DRI），即R/S（R为耐药细胞数，S为药敏细胞数）≥2为耐药，＜2为药敏。荧光指数（FI）=平均荧光强度×DRI，FI≥200为耐药，FI＜200为药敏。结果：初治5例AL中，4例为RSP，DRI=0.23～1.13，FI=50～160，诊断为药敏；另一例为LSP，RI=3.60，FI=367，诊断为原发耐药。15例临床耐药的AL中，12例为LSP，DRI=2.59～17.72，FI=265～1041，诊断为经典耐药；其余3例为RSP，DRI=0.39～0.67，FI=57～83，诊断为再生耐药。结论：用F检测白血病细胞内DNR的荧光直方图，DRI、FI临床耐药和耐药细胞荧光强度的诊断界点的方法，可以、简便、直观地在临床上和评价AL耐药的类型和程度，可为制定个体化疗的方案依据，也为观察逆转耐药的疗效了方法。 Signifiane f fluresent histgra f daunrubiin(DNR) in diagnsis f drug resistane t leukei ells 【Abstrat】bjetive：T develp a fluresent histgra f intraellular daunrubiin(DNR) as a diagnsti ethd fr drug resistane in aute yelid leukeia(AL).ethds：15 patients ith linially diagnsed drug resistant AL and 5 patients ith previusly untreated AL ere studied. The intensity f fluresene f intraellular DNR and the fluresent histgra ere deterined by fl yteter(F).Arding t a degree f the intensity, bth drug－resistan t and－sensitive znes ere arked ff. Left shift f ain peak(LSP) ith the fluresent histgra ay be diagnsed as drug resistane, and right shift f ain peak(RSP) as drug sensitivity. R/S std fr drug resistant index(DRI);R fr the nuber f drug resistant ells, and S fr drug－sensitive ells. DRI≥ 2 as drug－resistant r DRI ＜2 as drug-sensitive. Fluresent index(FI) as the prdut f ean fluresent intensity (FI) ultiplied by DRI. FI≥ 200 eant drug-resistant, hile FI＜ 200 eant drug-sensitive.Results：Ang 5 patients ith previusly untreated AL, 4 patients ere diagnsed as drug-sensitive fr RSP, DRI=0.23～ 1.13,FI=50～ 160,and ne patient as diagnsed as priary drug-resistant fr LSP,DRI=3.60, FI=367. In 15 patients ith linially drug resistant AL,12 patients ere diagnsed as lassial resistane fr LSP,DRI=2.59～ 17.72,and FI=265～