披碱草与野大麦杂交种BC1F2代同工酶分析.pdfVIP

披碱草与野大麦杂交种BC1F2代同工酶分析 李造哲 马青枝 内蒙古 农业 大学 ，内蒙 古呼 和浩 特 100019 摘要：采用不连续聚丙烯酰胺凝胶垂直平板电泳方法，对披碱草 （ElymusdahuricusTurcz.）、野 大麦 （HordeumbrevisubulatumHnk.）及其杂种BC1F2代分蘖期叶片的过氧化物酶(POD)和酯酶 （EST）同工酶进行了分析。结果表明：杂种BC1F2代的POD同工酶谱表现为双亲的互补类型， 杂种BC1F2代的EST同工酶谱亦主要表现为双亲的互补类型。杂种BC1F2代的POD和EST同 工酶谱中亲本的个别酶带有丢失现象，又分别产生了1条和7条杂种带。BC1F2代POD同工酶 谱的酶谱基本相同，EST酶带特征差异较大，出现双亲互补带和杂种带，并且有酶带的丢失现 象。BC1F2代的POD和EST同工酶谱明显地受轮回亲本野大麦的影响，说明回交使野大麦的 某些性状在BC1F2代进一步加强。聚类分析结果表明，株系Y1F2-4（6号）与Y1F2-5（7号） 及Y1F2-1（3号）与Y1F2-9(11号)有极其近的遗传关系，野大麦与BC1F2代各株系有较近的 遗传关系。POD与FST同工酶具多态性，可作为遗传标记用于杂种鉴定和目标性状植株的 检测。 关键词：披碱草；野大麦；过氧化物酶同工酶(POD)；酯酶同工酶(EST)；同工酶酶谱 AnalysisofPeroxidaseIsozymesandEsteraseIsozymesofElymusdahuricus, HordeumbrevisubulatumandTheirHybridBC1F2 LIZao-zheMAQin-zhi InnerMongoliaAgriculturalUniversity,Hohhot010019,China Abstract:Proxidase(POD)isozymesandesterase(EST)isozymesofElymusdahuricus,Hordeum brevisubulatumandtheirhybridF2andBC1F2wereanalyzedbydiscontinuitypolyacrylamidgelelectro phoresis.TheresultsshowedthatthePODandESTzymogramtypesofthehybridBC1F2weremainly themutualsupplementaryisozymesoftheparents.Someisozymebandsoftheparentswerelostinthe PODandESTzymogramtypesofthehybridBC1F2,thehybridBC1F2had1and5hybridbandsinthe PODandESTrespectively.TherewerenosignificantdifferencesinPODzymogramsamonghybrid BC1F2lines.TherewerevariationsintheESTzymograms,therewasacomplementarybandtypeofits parentsandveryfewisozymebandsoftheparentswerelost.ThezymogramsofBC1F2showedatend encytowardtherecurrentparentH.brevisublatumobviously,whichindicatedthatsomecharacteristics ofH.brevisubulatumwerestrengthenedinBC1F2bybackcross.Andtheresultsofclusteranalysis showedthattherewereclosegeneticrelationshipbetweenlineY1F2-4andlineY1F2-5,andbe tweenlineY1F2-1andlineY1F2-9.ThegeneticrelationsbetweenH.brevisubulatumandhybrid BC1F2werecloserthanthatbetweenE.d