盐酸非那吡啶大鼠体内药动学的研究.pdfVIP

′06 陕西药物分析学术研讨会 盐酸非那吡啶大鼠体内药动学研究 李强 刘佳 王嗣岑 陈琴华 (西安交通大学天然药物研究与工程中心 陕西 西安 710061) 摘要 目的：建立 RP-HPLC 测定盐酸非那吡啶在大鼠体内血药浓度的方法并进行药代动力 学研究。方法：液液萃取法浓集样品，外标法进行含量测定。色谱柱为 Diamonsil C18(4.6mm× 150mm，5μm) ，流动相为甲醇-水-三乙胺(60:39:1) ，流速 1.0mL/min ，检测波长 400nm 。 结果：盐酸非那吡啶在大鼠血浆中的最低定量限为 0.005μg/mL ，其高中低三个浓度的平均 提取回收率为 86.4%，日内及日间精密度均小于 10%；盐酸非那吡啶在大鼠血浆中达峰时间 0.36h，平均峰浓度 1.26μg/mL 。结论：分析方法灵敏，准确，适用于盐酸非那吡啶的药代动 力学研究。 关键词 盐酸非那吡啶；液液萃取；反相高效液相；药代动力学 Pharmacokinetics of Phenazopyridine Hydrochloride in rats by RP-HPLC Li Qiang, Liu Jia, Wang Si-cen, Chen Qin-hua (Institute of Pharmaceutical Research，Xi’an Jiaotong University) Abstract Objective: To establish an RP-HPLC method for the determination of phenazopyridine hydrochloride in rats’ blood plasma and to study its pharmacokinetics. Method: The blood samples were isolated by liquid-liquid extraction and analyzed by the RP-HPLC method. The samples were separated by Diamonsil C18 column, the mobile phase was Methanol-Water-Triethylamine (60:39:1), the flow rate was 1mL/min, and the detection wavelength was 400 nm. Results: The LLOQ of Phenazopyridine Hydrochloride in rats blood plasma was 0.005μg/mL. The calibration curve was linear in blood plasma over the concentration range of 0.005~3μg/mL(r=0.994). Average recovery added was 86.4%. Within-day and between-day precisions expressed as the relative standard deviation were below 10%. The parameters were Tmax= 0.36h, Cmax= 1.26μg/mL. Conclusion: The analytical method established in this study is stable and reliable, and can be used to measure the pharmacokinetics of Phenazopyridine Hydrochloride in rats. Key words: Phenazopyridine Hydr