脑源性神经营养因子靶向药物的制备和其对阿耳茨海默转基因鼠的治疗作用.pdfVIP

J ChinM edUniv 35 2 2006 4 # 113# 脑源性神经营养因子靶向药物的制备及其对阿耳茨海默 转基因鼠的治疗作用 1 1 1 2 2 1 王运杰 , 吴安华 , 杜江 ,任家谦 ,黄旭东 ,李 ( 1. , 110001; 2. ) [] : 检测脑源性神经营养因子( BDNF)靶向药物对 耳茨海默病 (AD)转基因鼠的治疗作用: 将 BDNF与抗 胰岛素受体单克隆抗体 ( 29B4) 相结合, 制备嵌合肽 BDNF-PEG-29B4/SA药物静脉注入 AD 转基因鼠 tg2576体内, 应用 ELISA方法检测AD转基因鼠脑内BDNF的粥样蛋白(AB )的含量: AD鼠脑内的 BDNF浓度较高和平稳可以有效降 低 AD转基因鼠脑内AB 含量: BDNF-PEG-29B4/SA 可以通过主动转运进入血脑屏障, 对 AD转基因鼠有治疗作用 [] 耳茨海默病;脑源性神经营养因子; 转基因鼠 [] 749. 16 [ ] A [] 0258- 4646(2006) 02- 0113- 02 P reparation of brain-derived neurotrophic factor targeting drug and its therapeutic effect in Alzheim er. s disease transgenic m ice 1 1 1 2 2 1 WANG Yun-jie, WU An-hua, DU Jiang, EN Jia-qian, HUANG Xu-dong, LI Zhe ( 1. Department ofN eurosurgery, TheFirst Affiliated Hospital, ChinaMedicalUniversity, Shenyang 110001, China; 2. Brain esearch Institute) [ Abstract] Objective: To prepare a targeting drug for brain-derived neurotrophic factor ( BDNF) and study its therapeutic effect in A lzhemi er. s disease (AD) transgenicm ice. M ethods: The chmi eric peptide (BDNF-PEG-29B4/SA ) was prepared by binding BDNF tomonoclonalantibody 29B4 and intravenously injected into AD transgenicm ice. The content ofB-amyloid protein (AB ) in the brains ofm icewas determ ined by using enzyme-linked mi munosorbent assay. Results: The concentration ofBDNF remained high and stable in the brains ofAD transgenicm ice. The targeting drug significantly decreased the content ofAB. Conclusion: BDNF targeting drug BDNF-PEG-29B4/SA can enter the blood-brain barrier by active transport and has a therapeutic effect inAD transgenic mice. [ Key words] A lzhemi er. s disease; brain-derived neurotrophic factor; transgenic mouse (Alzhemi er. s disease, AD) BDNF Amgen, ( 29B4mAb) EMD, B iosciences SA ( brain derived neuro- Sig