新生牛血清对大鼠脂肪来源细胞生长影响.pdfVIP

第34 卷第4 期 遵 义 医 学 院 学 报 Vo1.34 No.4 2011 年8 月 ACTA ACADEMIAE MEDICINAE ZUNYI Aug. 2011 新生牛血清对大鼠脂肪来源细胞生长的影响 李 萍，田 方 ，宋 琦，刘焕霞，李孟芳，魏 丹 （遵义医学院附属口腔医院 病例科，贵州遵义563000） [ ] 摘 要 目的探索新生牛血清对大鼠脂肪来源细胞生长状态的影响。方法 用新生牛血清代替传统的胎牛血清对脂 肪来源细胞进行体外培养，倒置相差显微镜下观察细胞形态，与胎牛血清培养条件下的脂肪来源细胞形态进行比较。 结果 新生牛血清培养条件下的大鼠脂肪来源细胞镜下呈短梭形或多角形，与胎牛血清培养的脂肪来源细胞形态相 似，但细胞在 P2~P3 代即出现了衰老趋势。结论 新生牛血清对体外培养的脂肪来源细胞的活力有负面影响，可能 不适用于脂肪来源细胞的培养。 [ ] 关键词 脂肪来源细胞；新生牛血清；细胞培养 [ ] [ ] [ ] 中图分类号 R78 文献标识码 A 文章编号 1000-27 15 （2011）04-0338-03 Effects of NBCS on the growth status of rat adipose tissue-derived cells LI Ping ，TIAN Fang ，SONG Qi ，LIU Huanxia LI Meng-fang ，WEI Dan （Affiliated Stomatological Hospital , Zuny i Medical College , Guizhou Zunyi 563000 , China) [Abstract] Obj ective To investigate the effect of NBCS (new-born calf serum) on the growth status of rat adipose tissue- derived cells. Methods SD rat groin fat tissue was isolated by enzyme digestion. Adipose tissue-derived cells were cultured ( ) with medium plused 10%NBCS instead of the traditionally plused 10% FBS fetal bovine serum . Cell morphology was as- sessed. Results Rat adipose tissue-derived cells under culture conditions with NBCS were observed by inverted phase-contrast microscope with short spindle and multi-angular forms, similar to the FBS culture conditions. But cells under culture con- ditions with NBCS tended to senesce during passage 2 to 3. Conclusions NBCS had negative effects to cell viability of the cultured rat adipose tissue-derived cells and may not apply to the culture of the rat adipose tissue-derived cells. [Key words] adipose tissue-der