白香丹对PMS肝气逆证模型大鼠不同脑区GABA_B受体亚基分布表达及海马ERK1%2f2信号通路的影响.pdfVIP

Effect of Baixiangdan Capsule on Location and Expression of GABAB Receptor subunits in Different Brain Regions and ERK1/2 Signal Transduction Pathway in Hippocampus of Premenstrual Syndrome Model Rats with liver-Qi invasion syndrome Specialty: Pharmacology of Traditional Chinese Medicine Tutor: Hui-yun Zhang Author: Qian Tan Abstract Objective:To investigate the changes between protein location and expression levels of γ-Aminobutyric Acid B1 Receptor and γ-Aminobutyric Acid B2 Receptor in Frontal cortex,Parietal cortex ,Hippocampus and Hypothalamus and the relevant protein expression levels of extracellular signal regulated protein kinase(ERK) signal transduction pathway in Hippocampus of Premenstrual Syndrome Model Rats with liver-Qi invasion syndrome, which gives a preliminary discussion on the pathogenesis of PMS with liver-qi invasion syndrome,as well as researches the intervention mechanism Baixiangdan Capsule for the disease. Methods: After open field test, macro-behavior observation and vaginal smear examination , we choose the Wistar rats of health and female into test, then build PMS model rats with liver-Qi invasion syndrome by electric stimulation,and evaluate all the group rats by open field test and macro-behavior observation. Then we adopt the immune fluorescence double-tagging technology to detect the protein distribution and expression levels of GABA R and GABA R in Frontal cortex,Parietal cortex ,CA1 and CA3 of B 1 B 2 Hippocampus and Hypothalamus in the normal group rats,model group rats , Baixiangdan treatment group rats and Baclofen treatment group rats,and calculate the mean fluorescence intensity in all group rats. And we use Western blot technology to detect t