鸭出血性卵巢炎毒对spf鸭和spf鸡的致病性研究.pdfVIP

摘 要 鸭出血性卵巢炎（Duck Hemorrhagic Ovaritis，DHO）是由新型黄病毒引起的一 种使感染鸭采食量下降并伴随产蛋率骤降为主要特征的传染病。本研究用 DHOV 感染 SPF 雏鸭和 SPF 鸡，建立出血性卵巢炎人工感染模型，通过研究其临床症状、病理学 变化、病毒抗原分布和定位、传播途径等内容，以明确该病的典型症状、病变特征和 发病机理，为养禽生产中防治该病提供试验依据。本试验分别将 F4 代 DHOV-HB 毒株 经口服途径对 12 只 34 日龄SPF 雏鸭和 11 只 120 日龄SPF 鸡进行攻毒，对照组动物 接种相同稀释度相同剂量的 SPF 鸭胚尿囊液。攻毒后观察动物的临床症状，并采集鼻 咽拭子接种鸡胚进行病毒分离，于感染后不同天数对SPF雏鸭和 SPF鸡进行病理解剖， 同时采取各种组织福尔马林固定、切片做病理组织学观察和病毒的免疫组化染色。结 果表明：SPF 鸭感染后典型症状：食欲不振，拉绿色稀便，精神萎靡，喜卧扎堆；眼 观病变为脾、肾明显肿大，肝表面有出血点；镜检病变：脾脏炎性细胞浸润，肝脏狄 氏腔显现，肝窦内皮细胞和枯否氏细胞肥大、活化增生，间质性肾炎，非化脓性脑炎， 胰岛溶解等。病毒在肝脏的病毒滴度最低。病毒抗原定位于多种细胞的细胞浆，脾脏 定位于淋巴细胞，肾脏定位于肾小管上皮细胞，肝脏定位于肝细胞，心脏定位于心肌 细胞，大脑定位于胶质细胞和神经细胞，胰腺定位于胰岛细胞，胸腺定位于网状细胞。 攻毒 SPF 鸡未出现明显的临床症状和病理变化，病毒分离均为阴性。本试验建立了 SPF 雏鸭 DHO 人工感染模型。 关键词：SPF 鸭；SPF 鸡；出血性卵巢炎；病理学；免疫组化 The Pathological Study of Duck Hemorrhagic Ovaritis Virus on SPF Ducks and SPF Chickens Abstract Duck Hemorrhagic Ovaritis(DHO) is a Infectious disease caused by a new type of flavivirus, making infected duck declined and had a rate of sudden drop in egg production as the main features. In this study, through infected SPF ducklings and SPF chickens with DHOV, we established the Duck Hemorrhagic Ovarian artificial infection model, study the clinical symptoms, the pathological changes, the distribution and positioning of the viral antigens, route of transmission and etc, in order to determine this disease typical symptoms, lesion characteristics and pathogenesis clearly, to provide the experimental basis for the prevention and control of disease in poulotry production. In this study, twelve SPF ducklings 34 days of age and eleven SPF chickens 120 days of age were inoculated by the oral route of F4 generation DHOV-HB strain respectively; Animals in the control group vaccinated duck embryo allantoi