电针内关对心肌缺血大鼠经穴皮肤组织中环磷酸鸟苷的影响.docVIP

电针内关对心肌缺血大鼠经穴皮肤组织中环磷酸鸟苷的影响 　　摘要：目的 观察大鼠心肌缺血损伤后，经穴皮肤组织中环磷酸鸟苷（cGMP）的特异性变化及电针“内关”对其的干予作用。方法 6只雄性Wistar大鼠作为正常组，18只大鼠制备心肌缺血后，分为模型组、高频组、低频组，高频组和低频组分别予100、2 Hz电针双侧“内关”，针刺时间20 min。治疗完毕后采用酶联免疫吸附法检测“内关”、“天泉”、“外关”及“足三里”等穴区皮肤组织中cGMP的含量。结果 心肌缺血模型组的“内关”、“天泉”、“外关”及“足三里”穴区cGMP含量较正常组均显著升高（P0.05或P0.001）；而高频或低频电针“内关”后，上述穴区cGMP含量较模型组均显著降低（P0.05或P0.001）。结论 高频或低频电针“内关”可保护大鼠心肌缺血损伤，其机制可能与降低心脏相关经脉穴区cGMP含量相关。 　　关键词：心肌缺血；电针；内关；环磷酸鸟苷；大鼠 　　DOI：10.3969/j.issn.1005-5304.2013.07.017 　　中图分类号：R245.3 文献标识码：A 文章编号：1005-5304（2013）07-0046-03 　　Effect on cGMP of Meridian Skin with Electroacupuncture on Myocardial Ischemia Rats DING Na， LI Xiao-hong， JI Bo， HU Lang-lin， SUN Zhi-fang， ZHU Shi-peng， LUO Li （School of Acupuncture-Moxibustion and Tuina， Beijing University of Chinese Medicine， Beijing 100029， China） 　　Abstract：Objective To observe the specific changes of content of cyclic guanosine monophosphate （cGMP） around meridian skin and specific influences of electroacupunture Neiguan （PC6） on cGMP of rats with acute myocardial ischemia （AMI）. Methods With 6 male Wistar rats as normal group， 18 rats were made AMI model and divided into model group， high-frequency group and low-frequency group （n=6）. The high-frequency and low-frequency group were given electroacupuncture in bilateral PC6 with 100 and 2 Hz for 20 min respectively. The content of cGMP in skin around PC6， Ximen （PC4）， Tianquan （PC2）， Waiguan （TE5） and Zusanli （ST36） was detected by using enzyme-linked immunosorbent method after treatment. Results In model group， cGMP content increased significantly around PC6， PC2， TE5 and ST36 compared with normal group （P0.05，P0.001）. After the treatment with high-frequency or low-frequency electroacupunture in PC6， cGMP content decreased significantly in skin around PC6， PC2， TE5 and ST36 compared with model group （P0.05， P0.001）. Conclusion High-frequency electroacupunture or high-frequency electroacupunture used in PC6 can protect rats from the injury of AMI. The mechanism may be related to