基质固相分散法同时提取净化烟草中多酚的研究+.pdfVIP

基质固相分散法同时提取净化烟草中多酚的研究 梁凤霞1蔡继宝1胡雅琴1谷勋刚2杨俊2苏庆德1 1(中国科学技术大学化学系，合肥230026) 30052) 2(中国科学技术大学烟草与健康研究中心，合肥2 摘要：研究建立了一种基质固相分散法同时提取净化烟草中多酚的分析方法。将烟草样品与反相 的同时提取和净化。应用该方法测定了烟草中多酚，回收率为91．7％．97．5％，相对标准偏差为 3．62％．6．31‰将该方法同加热回流方法进行了比较，研究结果表明：基质固相分散法的提取效率提 高10％．20％，有机溶剂用量减少1／2．1／3。 关键词：基质固相分散；多酚；烟草 onsimultaneousextractionand of Study cleanuppolyphenols 。 intobaccobasedonmatrixsolid phasedispersion LIANG Feng．xial，CAIJi．ba01，GU Jun2，SUQing．del’ Xun-gan92，YANG of ofScienceand of Chemistry,University China,Hefei，230026) 1(Department Technology CenterofTobaccoand ofScienceand of 2(Research Health，UniversityTechnologyChina,Hefei，230052) extractionand methodbasedonmatrixsolid for Abstract：Asimultaneous cleanup phasedispersion determinationof intobaccosWas tobacco andC18 sorbent polyphenols investigated．Dryground samples ina forminandthen a column．Elutionwith mortar5 into (1：4)were packedchromatogaphic homogenized a methanol weresimultaneousextractionand of 80％aqueoussolution，polyphenols cleanup．Therecovery were methodWas91．7％一97．5％．andtherelativestandarddeviations3．62％-6．3 propsed 1％．Compared with resultsof meth