子宫内膜异位症血瘀证患者在位内膜差异蛋白质组学研究.pdfVIP

- 22 一 ·临床研究· 南京中医药大学学报 2014 年 1 月第 30 卷第 1 期 ]aRNAL CF NAN]INJ UNT\伍RSIηCFRM\创二1) NJ ]an. aJ14 子宫内膜异位症血痕证患者在位内膜差异蛋白质组学 研究 黄金燕，文怡，魏绍斌铃 (成都中医药大学附属医院，四川成都 610072) 摘要:目的 通过蛋白纽学技术分离并鉴定子宫内膜异位症 (EMT)血痪证、气滞血亦证、肾虚血癖证患者在位内膜的差异蛋 白质，寻找 EMT 证候特异性蛋白质。方法 分别取 6 例子宫内膜异位症血痪证、气滞血必证、肾虞血惑证患者增生期在位内 膜组织和 6 例增生期正常子宫内膜组织标本，配对分为 6 纽。提取组织总蛋白，通过双向电泳技术分离总蛋白，并得到 6 纽双 向电泳凝胶图谱。通过 PDQuest7. 1. 1 软件对图像进行分析，并检测出表达差异点。通过基质辅助激光解吸电离飞行时间质 谱(MALDl-TOF-TOF/MS)及蛋白质数据库检索进行蛋白质鉴定。结果 根据蛋白质的不同等电点和分子量，通过双向凝 胶电泳和质谱分析技术可以分离人 EMT 患者及健康妇女在位内膜，发现 EMT 血癖证患者与健康妇女在位内膜之间有差异 蛋白表达，这些差异蛋白可能成为 EMT 发病的候选生物标志物。结论 EMT 血痪证、气滞血痪证、肾虚血痪证患者在位内 膜之间蛋白质组成分存在差异，这些差异可能是证候实质的内在根源。 关键词:子宫内膜异位症;血痪证;蛋白质量且学;双向电泳 p质谱 申困号 :R27 1. 9 文献标志码 :A 文章编号: 1672 - 0482( 2014) 01 - 0022 - 05 Eutopic Differential Proteomics Rωe盯ch on Endometriosis Patients with BIωd Stasis HUANG Jin-yan , WEN Yi , WEI Shao-Bin (Affiliated Hos户tal of Chengdu University of Chinese Medici肘 ， Chengdu , 610072 , China) ABSTACT: 0町ECTIVE To seperate EMT syndromes specific protein by isolating and identifying differences within eutopic protein in patients with EMT blood stasis syndrome , blood stasis due to qi stagnation , blood stasis due to kidney deficiency. METHODS The globle proteins of 6 endometrium tissue specimens of blood stasis syndrome in hyperplasia eutopic endometri- osis and the normal endometrium were extracted and divided into 6 couples. Total protein tissue was extracted , which was sep- arated by two-dimensional electrophoresis to attain 6 groups DE gel images. The image was analyzed through PDQuest7. 1. 1 and expression differences were detected. MALDI-TOF-TOF/MS was resoluted by matrix-assisted laser and protein was iden- tified in protein database. RESULTS ( According to isoelectric point and molecular weight of proteins , the differential ex- pressed endometriosis-associated protein of blood stasis syndrome and health women were separated and identified. And there were different expression of eutopic endometrium of three types of blood stasis syndrom