EHEC- O157 infection of renal cell apoptosis in mice.docVIP

 PAGE \* MERGEFORMAT 8 EHEC: O157 infection of renal cell apoptosis in mice On: Su Yuhong, Zhang Yang, Hong Yan Zhu, Zhao Wei, Pakistan phoenix [Abstract] Objective To detect and C3H/SW C3H/HeJ mice infected with EHEC (EHEC: O157) in renal cell apoptosis, and gene mutation of thl4 renal cell apoptosis. Methods Flow cytometry ( FCM) detection and C3H/SW C3H/HeJ mice infected with EHEC: O157 apoptosis of renal cells, the use of statistical software analysis SPSS11.0. Results of renal cell apoptosis in C3H/HeJ mice was significantly smaller than C3H/SW mice, the difference was statistically significant (P lt;0.05). Conclusion mice infected with EHEC: O157 thl4 of renal cell apoptosis and related gene mutation. [Keywords:] mice; EHEC: O157; renal cell; apoptosis; flow cytometry Abstract: Objective To detect apoptosis of renal cells in mice C3H/HeJ and C3H/SW infected with EHEC: O157 and to search for the effect of mutation of gene thl4 on apoptosis of renal cells.Methods The apoptosis of renal cells in mice infected with EHEC: O157 was detected by Flow Cytometry Method (FCM). The results were statistically analyzed by SPSS 10.0 software.Results The apoptosis rate of renal cells in C3H/HeJ mice was faster than that in C3H/SW mice. And the difference was significant . Conclusions The apoptosis of renal cells in mice infected with EHEC: O157 was associated with mutation of gene thl4. Keywords:: mouse; EHEC: O157; renal cells; apoptosis; Flow Cytometry Method Currently, renal cell apoptosis associated with renal disease is closely related to the detection of renal cell apoptosis can be used as an indicator of the diagnosis of many diseases, the monitoring body can resist further infection plays an important role. Task Force to use flow Type cytometry (Flow cytometry method, FCM) detection of gene mutation thl4 C3H/HeJ mice and normal mice C3H/SW content of Th1/Th2 cytokines and lymphocyte subpopulations of content, the above results show that the muta