Fluorescence in situ hybridization and immunohistochemical detection of breast cancer cerbB2 expression between.docVIP

 PAGE \* MERGEFORMAT 16 Fluorescence in situ hybridization and immunohistochemical detection of breast cancer cerbB2 expression between Of: Sheng Wei, Xiang-Ming, Shan Tao, Fan Lin, Li Meng, Zhang Qian, Tao Ghosh [Abstract] Objective To compare the immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) detection of breast cancer c erbB 2 state consistency. Methods A total of 50 cases of paraffin specimens of breast cancer, which was detected by IHC c erbB 2 protein expression (+) 10 cases, () 20 , () 20 cases. FISH method to detect the breast cancer cell c erbB 2 gene amplification, analysis and comparison between two methods and correlation. Results in IHC Detection of breast cancer c erbB 2 (+) and () when, FISH results with good agreement, the total coincidence rate was 89.2%. in IHC testing c erbB 2 () when, FISH results and their consistency over poor compliance rate was 35.3%. Conclusion IHC can be used as an initial screening of the expression of c erbB 2 preferred method, IHC detection of breast cancer c erbB 2 (+) and () when there is good agreement with the FISH. for IHC detection c erbB 2 expression () consistent with the FISH results is poor, these patients should be made in the application of FISH confirmed Herceptin. [Keywords:] breast cancer, c erbB 2, immunohistochemistry, fluorescence in situ hybridization ABSTRACT: Objective To compare the consistency of immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in detecting c erbB 2 status in breast cancer tissues. Methods A total of 50 breast cancer paraffin embedded samples were selected, of which there were 10 cases of c erbB 2 protein expression (+), 20 cases of () and 20 cases of (). FISH was used to assess the amplification of c erbB 2 gene, and SPSS 13.0 software was employed to analyze the difference and consistency between the two methods. Results IHC and FISH methods had a good consistency when detecting c erbB 2 (+) and () expressions in bre