Fluorescence Determination of berberine berberine hydrochloride tablets by.docVIP

 PAGE \* MERGEFORMAT 8 Fluorescence Determination of berberine berberine hydrochloride tablets by [Abstract] [Objective] To establish a fluorescence spectrophotometric determination of berberine hydrochloride tablets by the content of berberine. [Methods] fluorescence spectrophotometry with excitation wavelength of Ex = 365nm, emission wavelength under the condition Em = 409nm fluorescence intensity was measured. [Results] berberine 0.946μ g * ml-1 ~ 7.568μ g * ml-1 concentration range of linear relationship between the good, r = 0.9993. The average recovery was 97.51%, RSD = 1.15%. [Conclusion] The method is simple, rapid and accurate, can be used for the preparation of quality control. [Keywords:] fluorescence spectrophotometry; berberine hydrochloride tablets; berberine; assay Abstract: [Objective] To measure the content of berberine hydrochloride with fluorospectrophotometry. [Method] Take fluorospectrophotometry to measure the fluorescence strength at starting wave length Ex = 365nm, projected wave length Em = 409nm. [Result] At the concentration degree of 0.946μ g * mL-1 ~ 7.568μ g * mL-1, the hydrochloride had good linear relation, r = 0.9993.The average sample  adding rate of recovery was 97.51%, RSD = 1.15%. [Conclusion] The said method is simple for operation , with quick and correct result, can be used for quality control of the preparation. Keywords:: fluorospectrophotometry; berberine hydrochloride; content measurement Berberine hydrochloride tablets for the treatment of intestinal infection is a common antibacterial drugs, the main ingredient berberine. On its method for the determination of berberine hydrochloride: 2000 edition of ‘Chinese Pharmacopoeia’ used anti-titration method [1], 2005 edition of ‘Chinese Pharmacopoeia’ uses a high-performance liquid chromatography [1]. Reference to the relevant literature [3  5], established a fluorescence spectrophotometry determination of berberine hydrochloride tablets by means of berberin