Fluorescence detection of nitric oxide in seawater.docVIP

 PAGE \* MERGEFORMAT 13 Fluorescence detection of nitric oxide in seawater Of: Liu Chunying Zhao Ren Chunyan, Yang Guipeng Lipei Feng Han Yang [Abstract] based on 2,3-diamino naphthalene (DAN) and nitric oxide (NO) generated in the seawater fluorescence naphthol 2,3 triazole (NAT) to measure seawater NO. To determine the fluorescence method for the determination of NO conditions (λex = 383 nm, λem = 410 nm), temperature and time on the impact of NO DAN capture and DAN solution on trapping NO. measured NO concentrations and a linear relationship between fluorescence intensity and linear range of 1.4 ~ 1400 nmol / L, r = 0.9985, P lt;0.0001; detection limit of 1 nmol / L (S / N = 3); the relative standard deviation of 1.63% (14 nmol / L NO); no media interference. the same with NaOH under the conditions of the fluorescence method for the medium than the detection limit of this method will increase by 1 order of magnitude. with stones to purge and trap detection of the elderly in Qingdao (36 ° 05’46 “N; 120 ° 29’40” E) NO concentration in seawater (0.12 + -0.01) nmol / L, sodium nitroprusside can be used to monitor the release of NO in the law of the sea. off [Keywords:] Nitric oxide, water, fluorescence, 2,3-diamino naphthalene This article by the National Natural Science Foundation of China (Nos.40376022), Shandong Province, the Research Award Fund for Young Scientists (No. 2007BS08015), Shandong Province Natural Science Foundation (No.Y2008E04), National Outstanding Youth Science Foundation of China (No and Shandong Province Science and Technology Development Plan (No.2006GG2205024) funded projects 1 Introduction Nitric oxide (NO) is a special chemical properties but have significant environmental effects of free radicals, that is the important factor in ozone depletion. 80 years since the 20th century, the gradual development of molecular biology was discovered and confirmed in vivo NO important messenger molecule and the