Fluorescence quantitative PCR for screening of blood donors in the meaning of.docVIP

 PAGE \* MERGEFORMAT 3 Fluorescence quantitative PCR for screening of blood donors in the meaning of [Abstract] Objective: To explore the high-sensitivity fluorescence quantitative PCR screening of blood donors for HBV significance. Methods: 400 cases of blood donors negative for HBsAg ELISA method using fluorescence quantitative PCR specimens measured HBV  DNA were compared. Results: 400 specimens have five cases of HBV  DNA-positive, accounting for 1.25%. Conclusion: The fluorescence quantitative PCR for HBV screening of blood donors, compared with the ELISA method can effectively reduce the false negative and significantly improve the quality of blood and reduce the possibility of HBV transmission through blood transfusion. [Keywords:] blood donors; fluorescence quantitative PCR; Screening For blood transfusion safety, the state developed corresponding laws and regulations to manage, and blood donors were very strict screening. Now patients post-transfusion risk of infectious diseases has reached a very low level, but that does not mean that there is no risk, particularly in post-transfusion HBV infection, the risk is much higher than the risk of HCV and HIV infection, mainly Because China’s high HBV infection rate, viral mutation and the sensitivity of detection methods such as causes of false negatives caused by HBV. We are using fluorescence quantitative-PCR method on 400 cases of HBsAg ELISA-negative blood donors serum samples HBV  DNA testing, to explore the fluorescence quantitative PCR screening of blood donors in the significance of the results reported as follows. 1 Materials and methods 1.1 Blood samples all from Changsha City screening of eligible blood donors, a total of 400, HBsAg ELISA method negative. 1.2 fluorescence quantitative PCR reagent kit provided by daan genetic stock. 1.3 Instrument U.S. PE 5700 automatic fluorescence quantitative PCR instrument. 1.4 Methods HBV  DNA extraction: 50 μ l of serum DNA extra