Fluoride on the mouse amelogenin gene expression.docVIP

 PAGE \* MERGEFORMAT 17 Fluoride on the mouse amelogenin gene expression Of: Tao Hong, Wang Lin, Hou iron boat, Zhang Long, Xue-velvet [Abstract] Objective of fluoride on mouse amelogenin gene expression. Methods in the establishment of mice with moderate and severe dental fluorosis based on the model, in situ hybridization control (deionized drinking water), low fluoride groups (F-concentration of water 50mg / L), high fluoride group (F-concentration of water 150mg / L) mice during the development of the lower incisors of amelogenin mRNA in ameloblasts of proliferation and differentiation of ?p ?p mature secretory The expression of. Results of amelogenin mRNA in mouse ameloblast cell proliferation and differentiation of the expression is negative, the initial cells and the secretion of enamel matrix in the new expression began to appear weak; to the secretory phase, cells and enamel matrix in both neonatal showed strong positive expression; mature enamel of non-amelogenin mRNA expression. low fluoride group amelogenin enamel matrix protein mRNA in neonatal uneven surface of alternating strong and weak expression; fluoride group of amelogenin mRNA in the enamel matrix surface of newborn were positive and expression of enamel mineralization; in secretory ameloblasts, its expression many times interrupted. Conclusions of amelogenin mRNA fluoride in the ameloblasts, enamel matrix expression, inhibition of high concentrations of fluoride into the periodic ameloblasts secrete enamel matrix function, leading to incomplete mineralization of enamel, uneven and Focal mineralization defect. [Keywords:] fluorine; You of the original protein; in situ hybridization ABSTRACT: ObjectiveTo study the effect of fluorine on the amelogenin gene expression of mus musculus incisors.MethodsOn the basis of establishing a model for medium-and high-level dental fluorosis of mus musculus, this research analyzed the space-time expression of amelogenin in inferior incisor growt