HPLC determination of rhubarb extracts rhein microwave and the content of sennoside A.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of rhubarb extracts rhein microwave and the content of sennoside A [Abstract] Objective microwave rhubarb extracts rhein, two sennoside A Determination of the contents diarrhea. Methods using HPLC, column: Phenomenex Gemini C18 column (250 mm 4.6 mm, 5m, mobile phase : methanol 0.1% phosphoric acid (volume ratio of 80:20, flow rate: 1.0 mL / min, detection wavelength: 254 nm, column temperature: 25 . the results of determination of 3 batches of extract, rhein and sennoside average mass fraction of A 7.24 mg / g and 10.28 mg / g. Conclusion The method is simple and quick, reliable, and can be used as rhubarb extracts rhein and sennoside A method for the determination. [Keywords:] HPLC method, rhubarb extract, rhein, sennoside A Abstract: Objective To determine the contents of rhein and sennoside A in extracts of rhubarb prepared with microwave extraction. Methods Samples were analyzed on a Phenomenex Gemini C18 (250 mm 4.6 mm, 5 m) column using methyl alcohol 0.1% H3PO4 (80:20) as mobile phase. The flow rate was 1.0 mL / min and UV detection wavelength was 254 nm. The column temperature was 25 . Results Contents of rhein and sennoside A in the extracts of rhubarb were 7.24 mg / g and 10.28 mg / g, repectively. Conclusion The method is simple and reproducible, which could be used for the analysis of rhein and sennoside A in rhubarb extracts. Keywords:: HPLC, rhubarb extracts, rhein, sennoside A Rhubarb purgative anthraquinone constituents to sennoside A (sennoside A’s strongest diarrhea, rhein (rhein also be cathartic effect. In this paper, reference [1-3], based on the establishment of the determination of rhubarb extract in the microwave rhein and sennoside A purgative ingredients both the HPLC method for the study of rhubarb extracts from different extraction technologies, different varieties of rhubarb, rhubarb size of different parts of the role of the purgative composition analysis laid area ba