HPLC determination of two active ingredients aloe medicinal content.docVIP

 PAGE \* MERGEFORMAT 16 HPLC determination of two active ingredients aloe medicinal content 【Abstract】 Objective To establish a high-performance liquid chromatography sinensis Radix 6  hydroxy  2  [2  (4 ‘ methoxy-phenyl) ethyl] chromone (A), 6  hydroxy  2  (2  phenylethyl) chromone (B) 2 content of active ingredients. Methods HPLC, column was DiamonsilTMRP  18 column (250 mm × 4.6 mm, 5 μm); mobile phase of acetonitrile-methanol   water (27:27:46), flow rate 0.8 ml * min-1 ; column temperature 35 ℃; detection wavelength was 226 nm. Results 6  hydroxy  2  [2  (4 ‘ methoxy-phenyl) ethyl] chromone (A) at 0.070 8 ~ 0.354 mg and the peak area within the framework of a good linear relationship between the average recovery was 97.75 %, RSD of 1.14%; 6  hydroxy  2  (2  phenylethyl) chromone (B) at 0. 232 mg ~ 16 mg within the range of peak area showed good linear relationship between the average recovery was 98.40 %, RSD 1.29%. Conclusion The method is simple, accurate, and can be used for incense ingredients in 6 - hydroxy -2 - [2  (4 ‘ methoxy-phenyl) ethyl] chromone (A) and 6  hydroxy  2  (2  phenylethyl -yl) chromone (B) by HPLC. Keywords: Aquilaria 6  hydroxy  2  [2  (4 ‘ methoxy-phenyl) ethyl] chromone; 6  hydroxy  2  (2  phenylethyl) chromone; high performance liquid chromatography France Abstract: ObjectiveTo establish an HPLC method for the determination of 6  hydroxy  2  [2 - (4 ‘ methoxyl  phenyl) ethyl] chromone and 6  hydroxy  2  (2  phenylethyl) chromone in Aquilaria sinensis (Lour. ) Gilg.MethodsThe determination was performed on a DiamonsilTMRP-18 column. The mobile phase was acetonitrile-methanol-water solution (27:27:46) and UV detection wavelength was at 226 nm. The flow rate was 0.8 ml * min-1 and the column temperature was at 35 ℃. Results The linear ranges of 6-hydroxy  2  [2  (4 ‘ methoxyl  phenyl) ethyl] chromone and 6  hydroxy  2  (2  phenylethyl) chromone were 0. 0