HPLC determination of two small particles in the content of total flavonoids.docVIP

 PAGE \* MERGEFORMAT 7 HPLC determination of two small particles in the content of total flavonoids [Abstract] Objective To establish two small particles in the determination of total flavonoids. Methods The analytical column was Kromasil C18; methanol   water-acetic acid (45:53:2) as mobile phase; detection wavelength 359 nm; external standard method. The results of total flavonoids in 0.052 8 ~ 0.316 8 μ g / μ l within the linear relationship, Y = 16 714X-82.436, r = 0.999 4, the average recovery was 96.19%, RSD = 1.2%, (n = 6) . Conclusion The method is simple, sensitive, accurate, and can be used for quality control of two small particles. [Keywords:] total flavonoids; two small particle; high-performance liquid chromatography Abstract: ObjectiveTo establish a method for determination of the total flavonoid in Erding Granules.MethodsA Kromasil C18 column was used with methanol  water  acetic acid (45:53:2) as mobile phase.The content of ferulic acid was detected at a wavelength of 359 nm.ResultsThe linear range was 0.052 8 ~ 0.316 8 μ g (r = 0.999 4). The average recovery was 96.19% with RSD of 1.2% (n = 6). ConclusionThe method is simple, sensitive and accurate. It can be used to control the quality of Erding Granules. Keywords:: Total flavonoid; Erding Granules; HPLC 2 small particles from the Chinese violet, dandelion, Banlangen, lobelia herbs flavor composed of four Chinese herbal compound preparation, with Qingrejiedu, dampness, Tuihuang function. Toxic for hot boil carbuncle, sore throat, wind Heat eyes, damp-heat jaundice treatment. Income are set out in ‘The 2005 edition of Chinese Pharmacopoeia Ⅰ Division [1]. Was measured by HPLC method with rutin as a reference substance, the two dandelion small particles in the total content of flavonoids was determined. The method is simple and reproducible good. An instrument and reagent 1.1 Instrument Japan’s high-performance liquid chromatography system (Japan HITACHI series); UV757 UV-Vis