Human adipose stem cells to induce endothelial differentiation of Establishment of the best.docVIP

 PAGE \* MERGEFORMAT 13 Human adipose stem cells to induce endothelial differentiation of Establishment of the best Of: Yang Xufang Hexuhejian Mu Rui Zhang Lihong Zhang Li Yulin [Abstract] Objective To investigate the stem cells (human Adipose derived stem cells, hADSCs) to differentiate into endothelial cells in vitro induction system for the best. Methods collagenase digestion and adherence screening method were isolated from human adipose tissue, culture and amplification hADSCs, divided into 3 groups induced endothelial respectively: that of fibronectin (fibronectin, FN) coated group, gelatin coated group and did not shop group; also induce liquid divided into two groups: pure endothelial cell growth medium (EGM2 MV) group and endothelial cell growth medium (EGM2 MV) +50 ng / ml vascular endothelial growth factor (VEGF165) group. induced 15 d later, each group was detected by flow cytometry the surface of endothelial cell-specific expression of CD34 antigen; by cells observed by phase contrast microscope before and after induction of general morphology. Results Isolation, culture, a high level of homology hADSCs, CD29, CD44, CD90, CD105 and CD166-positive expression, while CD34, CD45 and HLA DR expression was negative; induced The results showed that the cells flow alone EGM 2MV 3 cells induced endothelial cell specific markers CD34 negative expression, and EGM2 MV +50 ng / ml VEGF165-induced cells, which express CD34 FN coated group was 8.4%, gelatin package was set at 5.4%, 4.2% group did not shop; difference can be seen under the microscope after the cells were induced by triangular or polygonal, integration at the cobblestone-like growth. Conclusion FN as the extracellular matrix and EGM2 MV +50 ng / ml VEGF165 composition synergistic effect induced by fluid, constitute hADSCs vitro differentiation into endothelial cells induced the best system for ischemic disease in clinical autologous cell transplantation therapy source of a large number of see