Human plasma asymmetric dimethylarginine concentrations of RP-HPLC assay.docVIP

 PAGE \* MERGEFORMAT 2 Human plasma asymmetric dimethylarginine concentrations of RP-HPLC assay [Abstract] Objective: To establish a human plasma-Africa Asymmetric Dimethylarginine (Asymmetric Dimethylarginine, ADMA) concentrations of RP-HPLC (RP-HPLC). Methods: Waters symmetry C18 column (5μ m, 3.90 mm * 150 mm), with 0.05 mmol / L sodium acetate (pH6.8) as mobile phase A, methanol as mobile phase B, linear gradient elution: 14% 15 min , 31% 25 min, 45% 5 min (V / V / V), flow rate 1.0 ml / min, fluorescence detection, excitation wavelength 330 nm, emission wavelength 450 nm, o-phthalaldehyde (OPA) to conduct pre-column derivatization at room temperature the next test. Results: ADMA in the range of 0.05 ~ 5μ g/ml there are a good linear relationship between the minimum detectable amount of 0.05μ g/ml, mean relative recovery was (93.76 ± 2.57)%, days, daytime average coefficient of variation, respectively (3.25 ± 1.59)%, (4.60 ± 1.37)%. Conclusion: This method is accurate, simple, rapid and applicable to scientific research and clinical testing. [Keywords:] asymmetric dimethylarginine chromatography RP-HPLC Plasma Asymmetric Dimethylarginine Central Africa (Asymmetrical dimethylarginine, ADMA) concentration and cardiovascular disease are closely related to the occurrence and development in recent years, studies have shown that elevated ADMA is a new risk factor for cardiovascular disease [1]. With regard to plasma ADMA concentration in detection methods, though there are already reports abroad [2,3], but the operation cumbersome and more interference factors. ROC using normal phase chromatography reported [4]. In this study, based on similar studies at home and abroad to improve the appropriate use of reversed-phase chromatography, with a linear gradient elution, establish a set of accurate, simple, rapid and high-performance liquid chromatography in human plasma ADMA concentration method, reported as follows. 1 Materials 1.1 reagent AMDA standard, deri