Panax notoginseng saponins on experimental diabetic cardiomyopathy rat heart glutathione S-transferase gene expression.docVIP

 PAGE \* MERGEFORMAT 9 Panax notoginseng saponins on experimental diabetic cardiomyopathy rat heart glutathione S-transferase gene expression [Abstract] Objective To investigate the PNS in experimental diabetic cardiomyopathy rat heart glutathione S-transferase (GST) gene expression. Methods 50 male successful model SD rats were randomly divided into treatment group 30 and 10 in the control group, gliclazide group 10. After 4 weeks, myocardial extraction of total RNA in each group by RT-PCR, with β-actin as an internal reference gene expression level of GST was detected. Results PNS group of different doses of GST gene expression increased, compared with the control group significantly (P lt;0.05). Conclusion PNS can induce GST gene expression, glutathione and pro-catalytic binding reaction occurs in Electronic , improving myocardial energy metabolism, reduce myocardial damage. [Keywords:] PNS; diabetic cardiomyopathy in rats; glutathione S-transferase enzymes; gene expression Diabetic cardiomyopathy (diabetic cardiomyopathy, DC) since 1974 and so was first proposed by the Hamby has been deep into the molecular etiology of genetic and gene level. Pathogenesis from its metabolism and mechanism of abnormal contraction, microcirculation, independent neuropathy and other point of view in-depth research. notoginseng saponins (PNS) is the main active ingredient of Chinese medicine Panax is an effective prevention and treatment of cardiovascular disease drug. In order to further investigate the PNS disease in diabetic cardiomyopathy Ultrastructure of rat heart and the role of genetic mechanisms on the molecular level, the experiment of the area. 1 Materials and equipment 1.1 Main reagents and equipment for the U.S. streptozotocin Sigma products, RNA extraction reagent Trizol Reagent were purchased from Sino-American Biotechnology Company, for Invitrogen products; reverse transcriptase (AMV-RT), ribonuclease inhibitor (RNasin) , dNTP, Taq DNA polymerase