Progress in animal models of liver injury.docVIP

 PAGE \* MERGEFORMAT 18 Progress in animal models of liver injury [Keywords:] liver disease, poison, carbon tetrachloride, acetaminophen, disease models, animal Liver injury is the result of various diseases of liver diseases, the prevention and treatment of liver injury is still a serious issue. Through the establishment of experimental liver injury in animal models to study the mechanism of liver disease, screening hepatoprotective drugs, liver function to explore the principle of has important practical significance. now at home and abroad in recent years, animal models of experimental liver injury classification, the role of principles, pros and cons of the modeling method and its research progress review and discussion. An animal model of liver injury 1.1 Carbon tetrachloride (carbon tetrachloride, CCl4 CCl4 liver injury caused major mechanism is that instead of their own and free radical related. CCl4 metabolism of free radicals into the body, in the liver via the cytochrome P450 activation, generates a triclosan hydroxyl radical and trichloromethyl peroxy radicals, attack the liver cell membrane phospholipid molecules, making the cell membrane, endoplasmic reticulum occurred chloride alkylation and lipid peroxidation, damage the cell membrane, organelles, and membrane lipids and also covalent binding of protein molecules to affect protein metabolism, and destroy the integrity of membrane structure and function, increase in calcium influx affect the normal function of cells, eventually leading to liver cell cytoplasm in soluble enzyme leakage, cell death [ 1]. CCl4 induced liver injury can be divided into acute and chronic. Acute liver injury: Lai Liying and other applications 4 mL / kg CCl4 dose of SD rats fed can induce acute liver failure, the mortality rate of 85% [2]. Chronic liver injury: Zhang and other use of 2 mL / kg CCl4 SD rats intraperitoneally, 2 times per week for 9 weeks can significantly associated with liver cell necrosis