Scorpion venom extract on leukemic mice E-cadherin CD49d and CXCR4 expression.docVIP

 PAGE \* MERGEFORMAT 16 Scorpion venom extract on leukemic mice E-cadherin CD49d and CXCR4 expression Of: Wen-Hua Yang, Lv Junxiu, history and philosophy of the new XD Yang, Gao Hong Tang Hao Zheng Yi Yu Wenjun [Abstract] Objective To investigate the scorpion venom extract (PESV of E-cadherin, CD49d and CXCR4 expression, explore PESV on leukemic cells and extramedullary infiltration. METHODS NOD-SCID mice 50, the establishment of human NOD-SCID mouse leukemia extramedullary infiltration were randomly divided into 5 groups of 10 each were PESV high, medium and low dose group, model group and control group. PESV high, medium and low dose group were intravenous injection of PESV 1.2,0.6,0.3 mg / kg, model group and control group were injected 0.9% saline 0.3 ml, 28 d after treatment, mice with peripheral blood flow cytometry the expression of CD49d and CXCR4, After 35 d the mice were killed by immunohistochemistry in liver was detected E-cadherin expression. CD49d and CXCR4 treatment group were lower than the model group the expression (P lt;0.01, each treatment group E-cadherin expression higher than model group (P lt;0.01. Conclusion PESV leukemia in mice can effectively improve the E-cadherin expression, lower expression of CD49d and CXCR4 has good extramedullary infiltration of leukemia cells by inhibition effect. [Keywords:] scorpion venom extract from leukemia E-cadherin CD49d CXCR4 The invasion process of malignant tumors, including: invasion, lymphatic permeation, vascular permeation, serosal and mucosal spread, with its transfer and adhesion molecule closely related. And leukemia infiltration of leukemic cells to the first escape from the bone marrow peripheral blood, then in turn by the chemotaxis and adhesion to vascular endothelium and produce migration, degradation of extracellular matrix, and then in extramedullary tissue survival, proliferation, invasion and the final formation of foci, the above process regulated by many factors, includ