Scutellaria antioxidant chemical constituents of.docVIP

 PAGE \* MERGEFORMAT 11 Scutellaria antioxidant chemical constituents of Authors: Shing-Yan, Liu Yu, Park Jin-hua, Park Kwang-Chun, Hui-son, Lee Ho 【Abstract】 Objective To study the skullcap (Scutellaria radix) of the chemical constituents and their antioxidant activity. Methods silica gel and reversed-phase silica gel column chromatography methods of separation, nuclear magnetic resonance spectroscopy methods such as structural identification, evaluation of antioxidant activity in vitro generation of bitterness diphenyl hydrazine (DPPH) radical scavenging experiments. Results were isolated from Scutellaria identified four flavonoid compounds: wogonin, baicalin yuan, 5, 7,2 ‘, 5’  4-hydroxy  8,6 ‘ dimethoxy-flavone and 5, 7, 2, ‘6’  4-hydroxy flavone, which baicalin yuan (compound 2) on the DPPH radical scavenging rate of 50% of the concentration (IC50) of 19 μmol / L. Conclusion Baicalein with anti-oxidative effect that baicalin has anti-oxidation, one of the major material components. Keywords: Scutellaria baicalensis Georgi; flavonoids; diphenyl hydrazide on behalf of the bitter Abstract: ObjectiveTo study the chemical constituents of Scutellaria and their antioxidant activity. MethodsSiliga gel and RP  C18 column chromatographies were used to isolate, and their structures were identified by spectral methods. Isolated compounds were tested in vitro for antioxidant activity against 1,1  diphenyl  2  picrylhydrazyl (DPPH) radical. ResultsFour known flavonoids were isolated from Scutellaria radix and their structures were identified as wogonin, baicalin, 5,7,2 ‘, 5’  tetrahydroxy  8,6 ‘ dimethoxyflavone and 5,7 , 2 ‘, 6’  tetrahydroxyflavone. Among these, baicalein had DPPH radical scavenging effect with IC50 value of 19 μmol / L. ConclusionBaicalin was isolated as the one of major flavonoids from Scutellaria Radix with antioxidation. Keywords:: Scutellaria; Flavonoid; DPPH scavenging Labiatae Scutellaria i