Secretions of patients with open fracture wounds and infections because of bacterial culture.docVIP

 PAGE \* MERGEFORMAT 15 Secretions of patients with open fracture wounds and infections because of bacterial culture Of: Bo so, Yang Zhe, Wang Dandan, Zhao Tao, Chen Wulin [Abstract] Objective To effectively prevent and control infection in open fractures, the authors collected 1320 cases of open fracture wounds in patients with secretions for bacterial culture, and the cause of the infection led to investigation and analysis. Methods (1) each patient 1 sample collected on day 2, 1st debridement before admission; 2nd debridement (or after surgery) 8 hours; (2) in the hospital after the first 2 to 5 days, depending on specific conditions and then collected samples of patients 1 or 2 times; (3) the submission of blood specimens were inoculated on agar plate, 37 C under aerobic conditions for 24 hours, after observation of colony morphology, Gram staining and biochemical test to identify bacterial species; (4 ) collection, analysis, recording relevant information (such as clinical information, laboratory data, etc.). Results 1320 cases of open fracture wound secretion specimens of patients were cultured and identified G-bacteria (accounting for 56.6%), G + bacteria ( 43.4%); in cultivated under aerobic conditions, specimens of 1016 cases of bacterial growth, “no bacterial growth,” the specimens of 304 patients; first admission, debridement debridement before and after 8 hours the number of two strains of the comparative samples, to @ = 0.05 as the test standard, the P lt;0.05, significant difference; hospital 8 hours after debridement, the lowest incidence of specimens from the specimen, the first 3 days the highest infection rate, length of hospitalization and infection rates are not positively correlated; The higher degree of open fracture injury, the higher infection rate. Conclusion G-bacilli as major pathogens orthopedic infections, open fractures for the prevention and control of infection, clinical response to anaerobic wound culture and other microb