Scutellaria the optimum conditions for enzymatic hydrolysis orthogonal filter.docVIP

 PAGE \* MERGEFORMAT 8 Scutellaria the optimum conditions for enzymatic hydrolysis orthogonal filter Keywords: orthogonal design;,, Huang Qin;,, enzymatic hydrolysis;,, baicalin Abstract: Objective To baicalin as an index to optimize Scutellaria the optimum conditions for enzymatic extraction. Methods filter enzymatic orthogonal design parameters. High performance liquid chromatography determination of baicalin, and calculate the yield. Results When the enzyme dosage of 1% substrate, insulation settling time of 1.5 h, extraction temperature 50 ℃, extraction time 2 h, the yield of baicalin 17.2%. Conclusion optimized hydrolysis conditions, the highest yield of baicalin. The study of baicalin extract preparation provides a new approach. Keywords: orthogonal design; Scutellaria baicalensis Georgi; digestion; baicalin The Optimum Extraction Technology of Baicalin from Radix Scutellaria by Enzymatic Hydrolysis Abstract: ObjectiveTo optimize the extraction process of baicalin from Scutellaria baicalensis on the condition of enzymatic hydrolysis.MethodsTo optimize by the orthogonal design, and to determine the content of baicalin by HPLC.ResultsThe optimum extraction condition was obtained as follows: the ratio of enzyme / substrate was 1%, the time of enzymatic hydrolysis was 2h at the extraction temperature of 50 ℃, and kept at 80 ℃ for 1.5h after adjusting the pH value to 2.0. ConclusionThe new method is developed for preparing the baicalin extract. Keywords:: Orthogonal design; Scutellaria baicalensis Geo.; Enzymatic hydrolysis; Baicalin Labiatae Scutellaria plants of Scutellaria baicalensis Geo.’s Dry root, bitter cold, with heat dampness, Xiehuo detoxification effect. Baicalin (baicalin) is the skullcap of the main active ingredient. Modern pharmacological studies have shown that: baicalin has Qingrejiedu, anti-inflammatory gallbladder, liver protection, anti-oxidation and anti-tumor and other functions, is widely u