Beneficial effect of reduced oxygen concentration with transfer of blastocysts in IVF patients older than 40 years old英文文献资料.docVIP

Vol.2, No.9, 1010-1017 (2010) Health doi:10.4236/health.2010.29149 Beneficial effect of reduced oxygen concentration with transfer of blastocysts in IVF patients older than 40 years old Javier I. García*, Soledad Sepúlveda, Luis Noriega-Hoces Laboratory of Assisted Reproduction, Concebir Clinic, Lima, Peru; *CorrespondingAuthor: jgarciaf@ Received 6 February 2010; revised 1 March 2010; accepted 5 March 2010. ABSTRACT ranging from 2 to 8% observed in atmospheric air [1-3]. This probably corresponds to an adaptation mechanism, as it is proven that higher oxygen concentrations may be harmful to the embryo [4] by generating reactive oxygen species (ROS) [5-7]. The aim of the present study was to determine the impact of oxygen concentration on implan- tation, pregnancy and delivery rates in IVF pa- tients older than 40 year old with transfer of blastocysts. Included were 558 women aged 23-45 years old undergoing IVF/ICSI procedures whose embryos were cultured at blastocyst stage under two different oxygen environments (a bi-gas system: 5.6% CO2 in air and a tri-gas system: 5.6% CO2, 5% de O2 and 89.4% N2). The main outcome measures of this study are im- plantation, pregnancy and delivery rates. Im- plantation, pregnancy and delivery rates are found to be reduced in women older than 40 years old. The implantation and pregnancy rates are significantly higher in women older than 40 years old from the 5% of O2 group, in compari- son to the 20% group (25.00% versus 2.70% and 41.38% versus 5.56%; P 0.05). The deliveries rates were 13.79% and 5.56% in the 5% and 20% oxygen groups respectively (P: NS). The birth- weight was similar in both study groups (P: NS). Gestational age was significantly longer in wo- men from the 5% of O2 group, in comparison to the 20% (36.87 versus 35.87 weeks, P 0.05). Results indicated that the embryonic culture with 5% of oxygen and transfer of blas