Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2′-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells英文文献资料.docVIP

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Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2′-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells英文文献资料.doc

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Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2′-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells英文文献资料

HindawiPublishingCorporation JournalofBiomedicineandBiotechnology Volume2010,ArticleID168306,9pages doi:10.1155/2010/168306 ResearchArticle BioimagingofNucleolinAptamer-Containing 5-(N-benzylcarboxyamide)-2-deoxyuridineMoreCapable ofSpeci?cBindingtoTargetsinCancerCells KyueYimLee, 1 HyunguKang, SungHoRyu, DongSooLee, 2 2 3 andSoonhagKim 1 JungHwanLee, 2 1 2 3 LaboratoryofMolecularImaging,DepartmentofAppliedBioScience,CHAStemCellInstitute,CHAUniversity, Seoul135-081,SouthKorea AptamerInitiative,POSTECHBiotechCenter,PohangUniversityofScienceandTechnology,Pohang, Kyungbuk790-784,SouthKorea DepartmentofNuclearMedicine,SeoulNationalUniversityCollegeofMedicine,Seoul110-744,SouthKorea CorrespondenceshouldbeaddressedtoSoonhagKim,kimsoonhag@ Received11June2009;Revised8September2009;Accepted19November2009 AcademicEditor:DaeheeKang Copyright?2010KyueYimLeeetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Chemicallymodi?ednucleotideshavebeendevelopedandappliedintoSELEXprocedureto?ndanoveltypeofaptamersto?t withtargetsofinterest.Inthisstudy,wedirectlyperformedchemicalmodi?cationof5-(N-benzylcarboxyamide)-2-deoxyuridine (called5-BzdU)intheAS1411aptamer,whichbindstothenucleolinproteinexpressedincancercells.Forty-sevencompounds ofAS1411-containingCy3-labeled5-BzdU(calledCy3-(5-BzdU)-modi?ed-AS1411)weresynthesizedbyrandomlysubstituting thymidines one to twelve in AS1411 with Cy3-labeled 5-BzdU. Both statistically quanti?ed ?uorescence measurements and confocalimaginganalysisdemonstratedatleastthreepotentialcompoundsofinterest:number12,29and41thatsigni?cantly increasedthetargetinga?nity tocancercellsbutnosigni?cantactivity fromnormalhealthycells.Theseresultssuggestthat thepositionandnumberofsubstituentsinAS1411arecriticalparameterstoimprovetheaptamerfunction.Inthisstudy,we demonstratedthatchemicalmodi?cati