Brazilian Green Propolis Protects against Retinal Damage In Vitro and In Vivo英文文献资料.docVIP

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Brazilian Green Propolis Protects against Retinal Damage In Vitro and In Vivo英文文献资料.doc

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Brazilian Green Propolis Protects against Retinal Damage In Vitro and In Vivo英文文献资料

eCAM2006;3(1)71–77 doi:10.1093/ecam/nek005 OriginalArticle BrazilianGreenPropolisProtectsagainstRetinalDamage InVitroandInVivo YutaInokuchi1,MasamitsuShimazawa1,YoshimiNakajima1,3,ShinsukeSuemori1,2, SatoshiMishima3andHideakiHara1 1DepartmentofBiofunctionalMolecules,GifuPharmaceuticalUniversity, 2DepartmentofOphthalmology, GifuUniversitySchoolofMedicineand 3ResearchCenter,APICo.Ltd,Gifu,Japan Propolis,ahoneybeeproduct,hasgainedpopularityasafoodandalternativemedicine.Itsconstituents havebeenshowntoexertpharmacological(anticancer,antimicrobialandanti-inflammatory)effects. WeinvestigatedwhetherBraziliangreenpropolisexertsneuroprotectiveeffectsintheretinainvitro and/orinvivo.Invitro,retinaldamagewasinducedby24hhydrogenperoxide(H2O2)exposure,and cellviabilitywasmeasuredbyHoechst33342andYO-PRO-1stainingorbyaresazurin–reductionassay. Propolisinhibitedtheneurotoxicityandapoptosisinducedinculturedretinalganglioncells(RGC-5,a ratganglioncelllinetransformedusingE1Avirus)by24hH2O2exposure.Propolisalsoinhibitedthe neurotoxicityinducedinRGC-5culturesbystaurosporine.Regardingthepossibleunderlyingmechan- ism,inpigretinahomogenatespropolisprotectedagainstoxidativestress(lipidperoxidation),asalso didtrolox(water-solublevitaminE).Inmiceinvivo,propolis(100mgkgà1;intraperitoneallyadmin- isteredfourtimes)reducedtheretinaldamage(decreaseinretinalganglioncellsandinthicknessof innerplexiform layer) induced byintravitreal in vivo N-methyl-d-aspartate injection. These findings indicatethatBraziliangreenpropolishasneuroprotectiveeffectsagainstretinaldamagebothinvitro andinvivo,andthatapropolis-inducedinhibitionofoxidativestressmaybepartlyresponsibleforthese neuroprotectiveeffects. Keywords:apoptosis–lipidperoxidation–NMDA–retinaldamage–retinalganglioncell Introduction haltorevenreversetheeffectofoculardiseasesareurgently needed.Hence,RGC deathand/oropticnervelossrepresent excellentneuroprotectivetargetsfordrugdiscovery(5). Propolis, a resinous substance made from the sprouts and bark