Brazilian Green Propolis Inhibits Inflammatory Angiogenesis in a Murine Sponge Model英文文献资料.docVIP

HindawiPublishingCorporation Evidence-BasedComplementaryandAlternativeMedicine Volume2011,ArticleID182703,7pages doi:10.1093/ecam/nep197 OriginalArticle BrazilianGreenPropolisInhibitsIn?ammatoryAngiogenesisin aMurineSpongeModel SandraAparecidaLimadeMoura, SilviaPassosAndrade, MariaLeticiaCostaReis, andDeniseCarmonaCara 1 M?onicaAlvesNevesDinizFerreira, MariadeLourdesNoviello, 1 1 2 1 1 1 2 DepartmentofGeneralPathology,InstituteofBiologicalSciences,FederalUniversityofMinasGerais(UFMG),BeloHorizonte,Brazil DepartmentofPhysiologyandBiophysics,InstituteofBiologicalSciences,FederalUniversityofMinasGerais(UFMG),BeloHorizonte, Brazil CorrespondenceshouldbeaddressedtoSilviaPassosAndrade,andrades@icb.ufmg.br Received11May2009;Accepted31October2009 Copyright?2011SandraAparecidaLimadeMouraetal.ThisisanopenaccessarticledistributedundertheCreativeCommons AttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalwork isproperlycited. Angiogenesis and in?ammation are persistent features of several pathological conditions. Propolis, a sticky material that honeybees collect from living plants, has been reported to have multiple biological e?ects including anti-in?ammatory and anti-neoplasicactivities.Here,weinvestigatedthee?ectsofwaterextractofgreenpropolis(WEP)onangiogenesis,in?ammatory cell accumulation and endogenous production of cytokines in sponge implants of mice over a 14-day period. Blood vessel formationasassessedbyhemoglobincontentandbymorphometricanalysisoftheimplantswasreducedbyWEP(500mgkg?1 orally)comparedtotheuntreatedgroup.Thelevelsofvascularendothelialgrowthfactor(VEGF)increasedprogressivelyinthe treatedgroupbutdecreasedafterDay10inthecontrolgroup.Accumulationofneutrophilsandmacrophageswasdeterminedby measuringmyeloperoxidase(MPO)andN-acetyl-β-D-glucosaminidase(NAG)activities,respectively. Neutrophilaccumulation was una?ected by propolis, but NAG activity was reduced by the treatment at Day 14. The levels TGF-β1