CD and DSC Investigation of Individual and Complex Influence of Meso-Tetra(4-Oxiethylpyridil) Porphyrin (TOEPyP4) and Its Zn-Complex on DNA英文文献资料.docVIP

American Journal of Analytical Chemistry, 2012, 3, 698-703 /10.4236/ajac.2012.310092 Published Online October 2012 (http://www.SciRP.org/journal/ajac) CD and DSC Investigation of Individual and Complex Influence of Meso-Tetra(4-Oxiethylpyridil) Porphyrin (TOEPyP4) and Its Zn-Complex on DNA Jamlet Monaselidze 1* , Evgeni Kiziria 1 , Maya Gorgoshidze 1 , David Khachidze 1 , Maya Kiladze 1 , Eteri Lomidze , Hakob Margaryan , Nune Hakobyan 1 2* 2 E. Andronikashvili Institute of Physics, Tbilisi State University, Tbilisi, Georgia Department of Radiophysics, Erevan State University, Erevan, Armenia 1 2 Email: j.monaselidze@aiphysics.ge, marhakob@ysu.am * * Received August 23, 2012; revised September 24, 2012; accepted September 30, 2012 ABSTRACT CD spectra of (DNA-TOEPyP4) + ZnTOEPyP4, (DNA-ZnTOEPyP4) + TOEPyP4, and DNA + (TOEPyP4-ZnTO- EPyP4) complexes have been studied. It is shown that CD spectra of these triple complexes significantly differ from the DNA-TOEPyP4 and DNA-ZnTOEPyP4 double complex spectra, and they are not sum of these double complexes. Es- pecially some strong differences in CD spectra of the triple and double complexes were observed when both porphyrins were added simultaneously into the DNA solution. In this case, ZnTOEPyP4 revealed a dominant influence on CD spectrum form. Zn-porphyrin also caused a strong intensity of positive band at 416 nm and a negative band at 437 nm when it was added into solution containing the DNA-TOEPyP4 complex. On the basis of obtained data, it was supposed that the observed significant changes in CD spectra of triple complexes were connected to an altered DNA conforma- tion initiated by intercalation of porphyrin TOEPyP4 into GC-rich sites. The melting process analysis of the double complexes was carried out.