Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis英文文献资料.docVIP
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Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis英文文献资料
HindawiPublishingCorporation
BiotechnologyResearchInternational
Volume2012,ArticleID817549,8pages
doi:10.1155/2012/817549
ResearchArticle
CellulosicEthanolProductionby
RecombinantCellulolyticBacteriaHarbouringpdcandadhII
GenesofZymomonasmobilis
P.SobanaPiriya,P.ThirumalaiVasan,V.S.Padma,U.Vidhyadevi,
K.Archana,andS.JohnVennison
DepartmentofBiotechnology,AnnaUniversityofTechnology,TamilNadu,Tiruchirappalli620024,India
CorrespondenceshouldbeaddressedtoS.JohnVennison,johnvennison36@
Received26March2012;Accepted12June2012
AcademicEditor:TriantafyllosRoukas
Copyright?2012P.SobanaPiriyaetal. This is an open access article distributed under the Creative Commons Attribution
License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly
cited.
Theethanol fermentinggenes such aspyruvate decarboxylase (pdc) andalcohol dehydrogenase II(adhII)were cloned from
Zymomonasmobilisandtransformedintothreedi?erentcellulolyticbacteria,namelyEnterobactercloacaeJV,ProteusmirabilisJV
andErwiniachrysanthemiandtheircellulosicethanolproductioncapabilitywasstudied.RecombinantE.cloacaeJVwasfoundto
produce4.5%and3.5%(v/v)ethanol,respectively,whenCMCand4%NaOHpretreatedbagassewereusedassubstrates,whereas
recombinantP.mirabilisandE.chrysanthemiwiththesamesubstratescouldonlyproduce4%,3.5%,1%,and1.5%ofethanol,
respectively.TherecombinantE.cloacaestrainproducedtwofoldhigherpercentageofethanolthanthewildtype.Therecombinant
E.cloacae straincouldbeimprovedfurtherbyincreasingitsethanoltolerancecapabilitythroughmediaoptimizationandalsoby
combiningmultigenecellulaseexpressionforenhancingethanolproductionfromvarioustypesoflignocellulosicbiomasssothat
itcanbeusedforindustriallevelethanolproduction.
1.Introduction
A suitable ethanologenic and cellulose-producing bacteria
could be developed by transferring genes that encode the
ethanol-fermentingenzymes[6]. Z.mobilisisoneofthebest
ethanol producers which produces ethanol in the Entner-
Doudoro?(ED)pathway,thati
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