RPS3a Over-Expressed in HBV-Associated Hepatocellular Carcinoma Enhances the HBx-Induced NF-κB Signaling via Its Novel Chaperoning Function 英文参考文献.docVIP

下载本文档

2
0
约5.97万字
约 17页
2017-05-13 发布于上海
举报
版权申诉

RPS3a Over-Expressed in HBV-Associated Hepatocellular Carcinoma Enhances the HBx-Induced NF-κB Signaling via Its Novel Chaperoning Function 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

RPS3a Over-Expressed in HBV-Associated Hepatocellular Carcinoma Enhances the HBx-Induced NF-κB Signaling via Its Novel Chaperoning Function 英文参考文献

RPS3aOver-ExpressedinHBV-AssociatedHepatocellular CarcinomaEnhancestheHBx-InducedNF-k BSignaling viaItsNovelChaperoningFunction Keo-HeunLim1,Kyun-HwanKim2,5*,SeongIlChoi7,Eun-SookPark2,SeungHwaPark4,KisunRyu1 ,Yong KwangPark2,SoYoungKwon3,Sung-IlYang2,5,HanChuLee6,In-KyungSung3,BaikL.Seong1,7* 1Department of Biotechnology, College of Life science and Biotechnology, Yonsei University, Seoul, Korea, 2Department of Pharmacology, IBST, Konkuk University SchoolofMedicine,Seoul,Korea,3DepartmentofInternalMedicine,IBST,KonkukUniversitySchoolofMedicine,Seoul,Korea,4DepartmentofAnatomyandCenterfor CancerResearchandDiagnosticMedicine,IBST,KonkukUniversitySchoolofMedicine,Seoul,Korea,5ResearchInstituteofMedicalSciences,KonkukUniversity,Seoul, Korea,6DepartmentofInternalMedicine,UniversityofUlsanCollegeofMedicine,AsanMedicalCenter,Seoul,Korea,7TranslationalResearchCenterforProteinFunction Control,YonseiUniversity,Seoul,Korea Abstract HepatitisBvirus(HBV)infectionisoneofthemajorcausesofhepatocellularcarcinoma(HCC)development.HepatitisBvirus Xprotein(HBx)isknowntoplayakeyroleinthedevelopmentofhepatocellularcarcinoma(HCC).Severalcellularproteins havebeenreportedtobeover-expressedinHBV-associatedHCCtissues,buttheirroleintheHBV-mediatedoncogenesis remains largely unknown. Here, we explored the effect of the over-expressed cellular protein, a ribosomal protein S3a (RPS3a),ontheHBx-inducedNF-kBsignalingasacriticalstepforHCCdevelopment.TheenhancementofHBx-inducedNF- kB signaling by RPS3a was investigated by its ability to translocate NF-kB (p65) into the nucleus and the knock-down analysis of RPS3a. Notably, further study revealed that the enhancement of NF-kB by RPS3a is mediated by its novel chaperoningactivitytowardphysiologicalHBx.Theover-expressionofRPS3asignificantlyincreasedthesolubilityofhighly aggregation-proneHBx.ThischaperoningfunctionofRPS3aforHBxiscloselycorrelatedwiththeenhancedNF-k Bactivity byRPS3a.Inaddition,themutationalstudyofRPS3ashowedthatitsN-terminaldomain(1–50