鄂西地区稻瘟病菌生理小种文库的构建及初步鉴定.pdfVIP

安徽农业科学，Joumal of Anhui A伊. Sci.2012 ,40(24) :12065 -12067 责任编辑金革责任校对李岩 鄂西地区稻瘟病菌生理小种文库的构建及初步鉴定 龙子文，周杰，玉春台，徐鑫 (中南民族大学生命科学学院，生物技术国家民委重点实验室，湖北武汉 43∞叫 摘要 [目的]构建鄂西地区的稻瘟病菌文库，并对其进行初步的鉴定分析。[方法]从鄂西地区 7 个水稻主产区不同的水稻品种上收 集稻瘟病感病稻籽，进行分离、纯化和菌落形态的初步鉴定分析，并利用 RAPD 和 REMAP 分子标记对该文库进行聚类分析。[结果]建 立了含有52 种菌株的稻瘟病菌生理小种文库;聚类分析结果表明，鄂西地区的稻瘟病菌群体结构呈现多样性和复杂性，遗传谱系与菌 落形态之间并不存在简单的一一对应关系。[结论]该研究为预测鄂西地区病原菌群体的变化趋势以及水稻抗病品种的选育，奠定了 基础。 关键词 稻瘟病菌;生理小种文库;鄂西地区:构建;鉴定 中图分类号 5432.4 文献标识码 A 文章编号 0517 -6611 (2012)24 -12065 -03 Construc出n and Ini创 Iden回ω咀on of Pbysiological Race Library of Ma伊， aporthe 0ηzea in W笛tem Hubei Pro由ce LONG Zi-wen et aI (Key Laboratory of State Ethnic Affairs Commission for Biological Technology , College of Life 5cience , 50uth-Central University for Nationalities , Wuhan , Hubei 43∞74) Abstract [0问ective] The aim was to construct and initially ident均由e physiological race librarγof Magnaporthe oryzea in western Hubei Province. [Method] M. oryzea infected rice straws were collected from 7 rice main production regions of westem Hubei Province. A ph均yslO阳0 logical 阻rac臼e libro缸j w圄 con崎s剑tructed 均by sin吨gle 叩spor阳e isolation , pu田ri面Iicωa础拍tion and identif泊fica础tion of colony morphology. Cluster analysis of 由1阻s li- brary w监 conducted 均byR阳AP阳D 四d REMAP. [R臼ult 1The results showed that a physiological race library of 52 isolates was constructed. Cluster analysis showed that population structure of M. oryzea demonstrated diversity and complexity , and there w田 no corresponding relation- ship between genetic lineages and colony mo甲hology. [Conclusion] The study provides a basis for predicting 由e trend of changes in M. oηzea population and breeding new resistant varieties in westem Hubei Province Key words Magnaporthe oryzea; Physiological race library; Western Hubei Province; Construction; Identification