血吸虫病门脉高压小鼠肠系膜血管病变与肠源性内毒素血症的关系.pdfVIP

· 1486· !年1 第24卷第12期 Chin J Exp Surg 。 December2007．v01 24．No．12 · 实 验 研 究 · 血吸虫病门脉高压小鼠肠系膜血管病变与 肠源性内毒素血症的关系 李岽健 杨镇 牛丽文 张爱龙 肖亮 【摘要】 目的 探讨血吸虫病门脉高压小鼠肠系膜血管病变，及其与肠源性内毒素血症之间 的相互关系。方法 将40只健康小鼠随机分成正常对照组(N组)20只与模型组(M组)20只，M 组采用腹部敷贴法制作血吸虫病模型，造模150 d后处死动物，鲎试验法测定血清内毒素水平；3色 染色和超微电镜观察肠系膜静脉血管形态学改变；逆转录聚合酶链反应和Western blot方法检测肠 系膜静脉血管中TLR-4、MD-2的表达。结果 M组血清内毒素水平高于N组(P0．01)；形态学 上M组肠系膜静脉血管出现明显的构型改变；M组小鼠肠系膜静脉血管TLR-4／MD-2表达水平高 于N组(P0．01)。结论 血吸虫病门脉高压时可发生明显的肠系膜血管病变；肠源性内毒素易 位、TLR-4／MD-2信号通路的激活是导致肠系膜血管病变产生的原因之一。 【关键词】 血吸虫病； 内毒素血症；血管病变 The relationship between inferior mesentetic vein vasculopahty and endotoxemia in rats with schis． tosomal cirrhosis L／Do g—J n，YANc Zhen，NlU Li—wen，et aL Department ofSurgery，Tongfi Hospi- tal，Tongfi Medical College，Huazhong Unwemi~ofScience and Technology，Wuhan 430030，China 【Abstract】 Objective To explore the relationship between inferior mesenteric vein vasculopathy and endotoxemia in rats with schistosomal cirrhosis．Methods Forty rats were divided into control grouD and model group．Twenty rats in model group were infected percutaneously with cercariae of SC stosomiasis iaponica．Quantitative analyses of Western blot and RT—PCR were applied to detect the protein and mRNA expression of TLR4／MD-2．Masson trichrome stain and transmission electron microscopic examination were performed to observe the pathologic changes of the infefior mesenteric vein．Vena cava blood was test． ed for endotoxin．Results The rats in model group had pathologic changes in inferior mesenteric vein．The expression of TLR_4／MD-2 in the infefior mesenteric vein in model group was significantly stronger than in control group(P0．O1)．The level of endotoxin in model group Was significantly higher than in model group(P0．O1)．Conclusion There iS an in