LC-MSMS法测定食用油中的辣椒素和二氢辣椒素.pdfVIP

2016 年第35 卷第7 期 中国酿造 总第 293 期 .175. 分析与检测 LC-MS/MS法测定食用油中的辣椒素和二氢躁椒素 梁桂娟，寻思颖*，张琼，李红洲 (贵州省产品质量监督检验院，贵州贵阳 55∞16) 摘 要:建立了用氨基固相萃取小柱净化提取液，液相色谱串联质谱CLC-MSIMS) 法测定食用泊中辣椒素和二氢辣椒素含量的检测 2 方法。结果表明，辣椒素在0~100ng抽L范围内线性关系良好CR =0.998 6) ，平均回收率在70.6%~90.5%，相对标准偏差CRSD， n=3) 2 在3.1%~8.9%，检出限为0.03μg/kg; 二氢辣椒素在0~20ng/mL范围内线性关系良好CR =0.998 8) ，平均回收率在71.5%~ 100.7%, 相对标准偏差CRSD， n=3) 在6.0%~ 13.2%，检出限为0.006μg/kg。该方法简便快速、准确可靠，是鉴别地沟油和测定食用泊中辣椒素 类物质的有效方法。 关键词:固相萃取:液相色谱串联质谱:食用油:辣椒素:二氢辣椒素 文章编号:0254-5071 (2016) 07-0175-05 doi:10.1188剖.issn.0254-5071.2016.07.038 中图分类号:0657.72 De阳mination of capsaicin and dihydrocapsaicin in edible oil by LC-MSIMS LIANG Guijuan, XUN Siying*, ZHANG Qio吨， LI Hongzhou (Gu泣bou Institute ofProduct Quality Supervision and De句口回nation， Guiyang 550016, China) Ab础部t: A method was established for the determination of capsaicin and dihydrocapsaicin in edible oil by solid phase extraction and LC-MSIMS. 2 The results indicated that the capsaicin showed good linear relationship in the range of 0-100 ng/ml (R =0.998 6), the average recovery was 70.6协90.5%， and relative standard deviation (RSD, n=3) was 3.1 %-8.9%, and 由e detection limit was 0.03μg/kg.ηle dihydrocapsaicin showed good linear relation- ship in the range ofO-20 n 2 g/ml (R =0.998 8), the average recovery was 7 1.5%-100.7% , relative stan也rd deviation (RSD, n=3) was 6.0%-13.2%, and 由.e detection limit was 0.006μg/kg. The method was simple, rapid ,